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Team:University of Ottawa/14 July 2008
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(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PCR Amplification''' ::<li> Corey decided it would be best to start from the beginning and amplify the PTP2 out of Genomic DNA again. I redid th...) |
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::<li> 97 plasmid was inoculated from master plate so that it can glycerol stocked for tomorrow. | ::<li> 97 plasmid was inoculated from master plate so that it can glycerol stocked for tomorrow. | ||
:'''PCR Confirmation''' | :'''PCR Confirmation''' | ||
| - | ::<li> Corey wanted me to do another PCR confirmation of the 97 plasmid with primers F58,F59. Expecting a band size of 2200 for this PCR. | + | ::<li> Corey wanted me to do another PCR confirmation of the 97 plasmid with primers F58,F59. Expecting a band size of 2200 for this PCR when I run it on a gel for tommorrow. |
Revision as of 19:14, 16 July 2008
Contents |
Today in the Lab
Matt
- PCR Amplification
- Corey decided it would be best to start from the beginning and amplify the PTP2 out of Genomic DNA again. I redid the PCR and I am running it overnight.
- Inoculation
- 97 plasmid was inoculated from master plate so that it can glycerol stocked for tomorrow.
- PCR Confirmation
- Corey wanted me to do another PCR confirmation of the 97 plasmid with primers F58,F59. Expecting a band size of 2200 for this PCR when I run it on a gel for tommorrow.
