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Team:University of Ottawa/15 July 2008
From 2008.igem.org
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:'''Inoculation of 97 - BY4742 cells''' | :'''Inoculation of 97 - BY4742 cells''' | ||
:<li> I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow | :<li> I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow | ||
| - | '''PCR confirmation''' | + | :'''PCR confirmation''' |
:<li> Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is. | :<li> Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is. | ||
| - | '''Digestion of PTP2''' | + | :'''Digestion of PTP2''' |
:<li> A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight. | :<li> A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight. | ||
Revision as of 19:27, 16 July 2008
Contents |
Today on the Lab
Tammy
- Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2
- 1 uL Pure pDR197:AtCKX2
- 1 ul 1:10 dilution pDR197:AtCKX2
- 1 ul 1:100 dilution pDR197:AtCKX2
- 100 ul XL10 E.Coli per reaction
- 900 ul LB Broth per reaction
- 3 LB Ampicillin (50 ug/mL) Plates
- Began Incubation of transformed cells at 1:50 pm.
Matt
- Inoculation of 97 - BY4742 cells
- I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
- PCR confirmation
- Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
- Digestion of PTP2
- A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.
