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Team:University of Ottawa/11 July 2008
From 2008.igem.org
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:'''PCR confirmation''' | :'''PCR confirmation''' | ||
::<li> PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success. | ::<li> PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success. | ||
| + | '''Chris''' | ||
| + | :'''Confirmation of AtCRE''' | ||
| + | ::<li> ran the 1% gel at 80 V for about forty minutes | ||
| + | ::<li> only primer bands showed after this time period | ||
| + | ::<li> it was suggested that phusion polymerase may not have been added before PCR, so 1 uL of phusion was added to the samples and phusion PCR was re-initiated | ||
| + | ::<li> following PCR, the results were run on a gel with the same results as above. | ||
Revision as of 19:50, 16 July 2008
Contents |
Today in the Lab
Matt
- PTP2 Digestion
- Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band sizes.
- PCR confirmation
- PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success.
Chris
- Confirmation of AtCRE
- ran the 1% gel at 80 V for about forty minutes
- only primer bands showed after this time period
- it was suggested that phusion polymerase may not have been added before PCR, so 1 uL of phusion was added to the samples and phusion PCR was re-initiated
- following PCR, the results were run on a gel with the same results as above.
