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20 May 2008
From 2008.igem.org
(New page: ==To Do== Today we are researching PIF3 degredation as it might occur in Ecoli Writing the new fundraising letters Finishing construction of our biobricks Colony PCR,Gel extraction, a...) |
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Colony PCR,Gel extraction, and streaking positive colonies and setting up O/N cultures of last years project. | Colony PCR,Gel extraction, and streaking positive colonies and setting up O/N cultures of last years project. | ||
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| + | <u>'''Chris'''</u><br> | ||
| + | - Ran the thiolase sequencing that I did last week up to the MBSU. We should get the results back tomorrow. | ||
| + | |||
| + | - Finished up the BioBricks we've worked on so far: PIF3 and the Phytochromobilin/Heme Oxygenase Operon. The HIS-tags have been added to all three genes and they have been optimized for expression in ''E.coli'' (strain K12, specificly, though we are going to be using XL10. The optimization site I mentioned in the May 16th notes did not have the XL10 strain listed so I went with K12. I don't anticipate this being a problem). The | ||
| + | Phytochromobilin/Heme Oxygenase operon looks like this:<br> | ||
| + | [[Image:phy_operon.png]]<br> | ||
| + | The black squares at either end are the standard BioBrick suffix and prefix. The blue represents a terminator sequence (part BBa_B1001) and the red diamonds mark where we put in the RBS (part BBa_B0034). | ||
==Lab Tip of The Day== | ==Lab Tip of The Day== | ||
'''If you can't log into a MAC computer DONT put you CD in it as you will not be able to retrive it''' | '''If you can't log into a MAC computer DONT put you CD in it as you will not be able to retrive it''' | ||
Revision as of 19:54, 20 May 2008
To Do
Today we are researching PIF3 degredation as it might occur in Ecoli
Writing the new fundraising letters
Finishing construction of our biobricks
Colony PCR,Gel extraction, and streaking positive colonies and setting up O/N cultures of last years project.
Chris
- Ran the thiolase sequencing that I did last week up to the MBSU. We should get the results back tomorrow.
- Finished up the BioBricks we've worked on so far: PIF3 and the Phytochromobilin/Heme Oxygenase Operon. The HIS-tags have been added to all three genes and they have been optimized for expression in E.coli (strain K12, specificly, though we are going to be using XL10. The optimization site I mentioned in the May 16th notes did not have the XL10 strain listed so I went with K12. I don't anticipate this being a problem). The
Phytochromobilin/Heme Oxygenase operon looks like this:
The black squares at either end are the standard BioBrick suffix and prefix. The blue represents a terminator sequence (part BBa_B1001) and the red diamonds mark where we put in the RBS (part BBa_B0034).
Lab Tip of The Day
If you can't log into a MAC computer DONT put you CD in it as you will not be able to retrive it
