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Team:ESBS-Strasbourg/16 July 2008
From 2008.igem.org
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* Gel of the PCR from yesterday | * Gel of the PCR from yesterday | ||
* Transformations | * Transformations | ||
| - | ** '''cells | part/vector | protocol'''< | + | **'''cells | part/vector | protocol''' |
| - | dh5a (Mariel) | pxj (10 ng) | HeatShock (Maria) | + | <pre> |
| - | + | dh5a (Mariel) | pxj (10 ng) | HeatShock (Maria) | |
| - | + | = | = | KCM (Barbara) | |
| - | + | = | LexA-BD (1 µl) | = | |
| - | + | = | BB_pSB (2 µl) | = | |
| - | dh5a (ours) | pxj (10 ng) | HS | + | = | BB_1763004 (2 µl)| = |
| - | Top10 | = | = | + | dh5a (ours) | pxj (10 ng) | HS |
| - | db3.1 | = | = | + | Top10 | = | = |
| - | + | db3.1 | = | = | |
| + | = | BB_p1010 (2 µl) | = | ||
| + | </pre> | ||
<br> | <br> | ||
Mariel repeats the PCR from Paul from yesterday with 10ng plasmids <br> | Mariel repeats the PCR from Paul from yesterday with 10ng plasmids <br> | ||
Revision as of 08:14, 17 July 2008
Contents |
DryLab
michi:
- IRESes s*** let's mutate our promoter
- last chance for IRES: wrote email to Pospisek
- Calculated size of our constructs: 6 kb (too big for vector?)
Wiki
- Further content in all categories (MM)
Modeling
WetLab
Sandra:
- Gel of the PCR from yesterday
- Transformations
- cells | part/vector | protocol
dh5a (Mariel) | pxj (10 ng) | HeatShock (Maria)
= | = | KCM (Barbara)
= | LexA-BD (1 µl) | =
= | BB_pSB (2 µl) | =
= | BB_1763004 (2 µl)| =
dh5a (ours) | pxj (10 ng) | HS
Top10 | = | =
db3.1 | = | =
= | BB_p1010 (2 µl) | =
Mariel repeats the PCR from Paul from yesterday with 10ng plasmids
=>Amplification of Gal4 succeed
Paul:
- Purification of Gal4 PCR product, stored at -20°C
