Team:ESBS-Strasbourg/TENS

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(New page: =TENS= Tris-HCl 10 mM pH8<br> EDTA 1 mM pH8<br> NaOH 0.1 M<br> SDS 0.5%<br> Make a Sterilfiltration of the solution or use steril stock solutions for its preparation '''For 40 mL of TEN...)
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Revision as of 09:49, 17 July 2008

TENS

Tris-HCl 10 mM pH8
EDTA 1 mM pH8
NaOH 0.1 M
SDS 0.5%

Make a Sterilfiltration of the solution or use steril stock solutions for its preparation

For 40 mL of TENS:

  • 0.8 mL of 0.5M Tris-HCL pH8
  • 0.1 mL of 0.4M EDTA
  • 0.4 mL of 10 M NaOH
  • 1 mL of 20% SDS
  • fill up to 40 mL with milliQ <math>H_20</math>