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Team:ESBS-Strasbourg/TENS
From 2008.igem.org
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Make a Sterilfiltration of the solution or use steril stock solutions for its preparation | Make a Sterilfiltration of the solution or use steril stock solutions for its preparation | ||
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'''For 40 mL of TENS:''' | '''For 40 mL of TENS:''' | ||
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*1 mL of 20% SDS | *1 mL of 20% SDS | ||
*fill up to 40 mL with milliQ water | *fill up to 40 mL with milliQ water | ||
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[[Team:ESBS-Strasbourg/Protocols|''Protocols'']] | [[Team:ESBS-Strasbourg/Protocols|''Protocols'']] | ||
Revision as of 09:54, 17 July 2008
Tris-HCl 10 mM pH8
EDTA 1 mM pH8
NaOH 0.1 M
SDS 0.5%
Make a Sterilfiltration of the solution or use steril stock solutions for its preparation
For 40 mL of TENS:
- 0.8 mL of 0.5M Tris-HCL pH8
- 0.1 mL of 0.4M EDTA
- 0.4 mL of 10 M NaOH
- 1 mL of 20% SDS
- fill up to 40 mL with milliQ water
