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Team:ESBS-Strasbourg/16 July 2008
From 2008.igem.org
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Paul: | Paul: | ||
* Purification of Gal4 PCR product, stored at -20°C | * Purification of Gal4 PCR product, stored at -20°C | ||
| + | <br> | ||
| + | Katja: | ||
| + | *Preparation of TENS buffer (not steril filtered) | ||
| + | *Preparation of 3M NaAcO pH5 | ||
| + | *Miniprep with b.1) Protocol of the BBa_J63002(?) Plasmid to verify the identity by digestion after PCR failed | ||
== General == | == General == | ||
Latest revision as of 10:11, 17 July 2008
Contents |
DryLab
michi:
- IRESes s*** let's mutate our promoter
- last chance for IRES: wrote email to Pospisek
- Calculated size of our constructs: 6 kb (too big for vector?)
Wiki
- Further content in all categories (MM)
Modeling
WetLab
Sandra:
- Gel of the PCR from yesterday
- Transformations
- cells | part/vector | protocol
dh5a (Mariel) | pxj (10 ng) | HeatShock (Maria)
= | = | KCM (Barbara)
= | LexA-BD (1 µl) | =
= | BB_pSB (2 µl) | =
= | BB_1763004 (2 µl)| =
dh5a (ours) | pxj (10 ng) | HS
Top10 | = | =
db3.1 | = | =
= | BB_p1010 (2 µl) | =
Mariel repeats the PCR from Paul from yesterday with 10ng plasmids
=>Amplification of Gal4 succeed
Paul:
- Purification of Gal4 PCR product, stored at -20°C
Katja:
- Preparation of TENS buffer (not steril filtered)
- Preparation of 3M NaAcO pH5
- Miniprep with b.1) Protocol of the BBa_J63002(?) Plasmid to verify the identity by digestion after PCR failed
General
Our account is open again.(KK)
