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Minnesota/17 July 2008
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|1. '''Plasmid prep dual promoters:''' (1) Tet & P22mnt, (2) LacI & LAMBDAcI. | |1. '''Plasmid prep dual promoters:''' (1) Tet & P22mnt, (2) LacI & LAMBDAcI. | ||
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| - | |2. ''' | + | |2. '''Model:''' Figure out why have such a low GFP output when run model. |
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|3. '''Sequence L5, L6-L9''' | |3. '''Sequence L5, L6-L9''' | ||
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|5. '''Discuss problems with sequences''' | |5. '''Discuss problems with sequences''' | ||
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| - | |6. ''' | + | |6. '''Double digest of all ligations:''' from previous days. Do a double digest to cut the components linearly, which will then be able to run through a gel to check if correct DNA is present. Follow the table below: |
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| + | !| Parts !! 10x Buffer !! BSA !! H20 !! DNA !! RE 1 !! RE 2 | ||
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| + | |L2a, LAMBDAcI:Term ||5.0uL ||0.5uL ||32.5uL ||10.0uL ||EcoRI ||Xba1 | ||
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Revision as of 19:16, 17 July 2008
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| 1. Plasmid prep dual promoters: (1) Tet & P22mnt, (2) LacI & LAMBDAcI. | |||||||||||||||
| 2. Model: Figure out why have such a low GFP output when run model. | |||||||||||||||
| 3. Sequence L5, L6-L9 | |||||||||||||||
| 4. Re-transform RFP, TetR promoter, terminator because no cell growth on plates. | |||||||||||||||
| 5. Discuss problems with sequences | |||||||||||||||
6. Double digest of all ligations: from previous days. Do a double digest to cut the components linearly, which will then be able to run through a gel to check if correct DNA is present. Follow the table below:
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