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Team:The University of Alberta/17 July 2008
From 2008.igem.org
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*Did Minipreps of I0500 in the new vector: Now we have I0500 in a high copy plasmid! No more adding IPTG to switch it from low-copy to high-copy mode! | *Did Minipreps of I0500 in the new vector: Now we have I0500 in a high copy plasmid! No more adding IPTG to switch it from low-copy to high-copy mode! | ||
*More Colony PCR on the synthed biobricks. The final one has arrived! | *More Colony PCR on the synthed biobricks. The final one has arrived! | ||
| - | *Did sequencing for BisDA to cofirm whether it is the part, because the colony PCR was not as expected. | + | *Did sequencing for BisDA to cofirm whether it is the part, because the colony PCR was not as expected. '''EDIT''': The colony PCR was fine; parts were a bit larger than we had thought because we did not account for the MCS being PCR'd along with the parts because of the primers we used. |
| + | *Digested Tryp with Eco/Spe (sequential digest; first with Spe and then with Eco) and J6 with Eco/Xba. This will allow us to ligate the two together. | ||
Revision as of 21:18, 17 July 2008
Today
- Did Minipreps of I0500 in the new vector: Now we have I0500 in a high copy plasmid! No more adding IPTG to switch it from low-copy to high-copy mode!
- More Colony PCR on the synthed biobricks. The final one has arrived!
- Did sequencing for BisDA to cofirm whether it is the part, because the colony PCR was not as expected. EDIT: The colony PCR was fine; parts were a bit larger than we had thought because we did not account for the MCS being PCR'd along with the parts because of the primers we used.
- Digested Tryp with Eco/Spe (sequential digest; first with Spe and then with Eco) and J6 with Eco/Xba. This will allow us to ligate the two together.
