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Minnesota/18 July 2008
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''Solution:'' Re-transform (again) paper DNA with MCherry, RFP, Term., and TetR. | ''Solution:'' Re-transform (again) paper DNA with MCherry, RFP, Term., and TetR. | ||
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| - | |3. Double Digest: Double digesting dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP. RFP will not be used because it has not been properly transformed yet. | + | |3. '''Double Digest:''' Double digesting dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP. RFP will not be used because it has not been properly transformed yet. |
Revision as of 15:42, 18 July 2008
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| 1.Analyze gel results from 07-17-2008: Send in select samples from the gel for sequencing. Send in reaction mixture containing template DNA and primers that will bind to it, and thus allow to sequence. Sequencing L3i, L3j, L4b, L4i, L5c, L6a, L6b, L6d, L6e, L7a, L7b, L7d, and L8a. |
| 2. Problem: No growth on plates with MCherry, RFP, Terminator, and TetR promoter.
Solution: Re-transform (again) paper DNA with MCherry, RFP, Term., and TetR. |
| 3. Double Digest: Double digesting dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP. RFP will not be used because it has not been properly transformed yet. |
