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Minnesota/18 July 2008
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|'''3. Double Digest and Ligate:''' Double digesting then ligating dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP. | |'''3. Double Digest and Ligate:''' Double digesting then ligating dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP. | ||
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| + | !| Parts !! 10x Buffer !! BSA !! H20 !! DNA !! RE 1 !! RE 2 | ||
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RFP will not be used because it has not been properly transformed yet. Will result in: | RFP will not be used because it has not been properly transformed yet. Will result in: | ||
Revision as of 21:24, 18 July 2008
| Back to Notebook Home | |
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| 1. Analyze gel results from 07-17-2008: Send in select samples from the gel for sequencing. Send in reaction mixture containing template DNA and primers that will bind to it, and thus allow to sequence. Sequencing L3i, L3j, L4b, L4i, L5c, L6a, L6b, L6d, L6e, L7a, L7b, L7d, and L8a. | |||||||||||
| 2. Problem: No growth on plates with MCherry, RFP, Terminator, and TetR promoter.
Solution: Re-transform (again) paper DNA with MCherry, RFP, Term., and TetR. | |||||||||||
3. Double Digest and Ligate: Double digesting then ligating dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP.
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