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21 May 2008
From 2008.igem.org
(Difference between revisions)
Etphonejoe (Talk | contribs) (→Volunteers Scheduled for Today) |
Etphonejoe (Talk | contribs) (→Volunteers Scheduled for Today) |
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5-8 AS<br> | 5-8 AS<br> | ||
| - | 6-8 DL | + | 6-8 DL<br> |
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Things to do:<br> | Things to do:<br> | ||
Mini-preps of O/N's<br> | Mini-preps of O/N's<br> | ||
Revision as of 15:16, 21 May 2008
Volunteers Scheduled for Today
5-8 AS
6-8 DL
Things to do:
Mini-preps of O/N's
Sequence gel purified fragments from yesterday
Transform I0500
Digest J61003 with Xba/Pst to insert ORF's
Digest minipreps with Xba/Pst to insert into J61003
Run gel of digests and excise correct fragments
Ligate vector/inserts
Transform ligation reactions
Lab clean up
