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Minnesota/21 July 2008
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| - | |1. Results from 07-18-2008: Only terminator growth. RFP, MCherry, and | + | |1. '''Results from 07-18-2008:''' Only terminator growth. RFP, MCherry, and TetR promoter had no growth. Make 2mL cultures of terminator in LB media - once have growth after 2 hrs with shaking @ 220rpm's, then make 5mL cultures. The 5mL cultures are composed of 4.5mL LB media and 0.5mL from each 2mL cultures. |
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| 1. Results from 07-18-2008: Only terminator growth. RFP, MCherry, and TetR promoter had no growth. Make 2mL cultures of terminator in LB media - once have growth after 2 hrs with shaking @ 220rpm's, then make 5mL cultures. The 5mL cultures are composed of 4.5mL LB media and 0.5mL from each 2mL cultures. |
