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Minnesota/21 July 2008
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|'''1. Results from 07-18-2008:''' Only terminator growth. RFP, MCherry, and TetR promoter had no growth. Make 2mL cultures of terminator in LB media - once have growth after 2 hrs with shaking @ 220rpm's, then make 5mL cultures. The 5mL cultures are composed of 4.5mL LB media and 0.5mL from each 2mL cultures. | |'''1. Results from 07-18-2008:''' Only terminator growth. RFP, MCherry, and TetR promoter had no growth. Make 2mL cultures of terminator in LB media - once have growth after 2 hrs with shaking @ 220rpm's, then make 5mL cultures. The 5mL cultures are composed of 4.5mL LB media and 0.5mL from each 2mL cultures. | ||
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| - | |'''2. Go to St. Paul:''' Get BioBrick parts from iGEM notebook - (1)E0030, YFP (2) J06504, RFP (3) R0040, TetR Promoter | + | |'''2. Go to St. Paul:''' Get BioBrick parts from iGEM notebook - (1)E0030, YFP (2) J06504, RFP (3) R0040, TetR Promoter (4) I732077, GFP + LVA (5) E0032, YFP+LVA and (6) I732078, RBS+RFP+LVA+Term. |
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|'''3. Order the following:''' (1) Dual promoter tailed primers to remove RBS, and (2) SSRA tag + Restriction sites tailed primers for GFP and YFP/RFP. | |'''3. Order the following:''' (1) Dual promoter tailed primers to remove RBS, and (2) SSRA tag + Restriction sites tailed primers for GFP and YFP/RFP. | ||
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|'''4. Biomedical Genomics Center:''' Contact them for sequencing problems to target the issue. | |'''4. Biomedical Genomics Center:''' Contact them for sequencing problems to target the issue. | ||
Revision as of 14:58, 21 July 2008
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| 1. Results from 07-18-2008: Only terminator growth. RFP, MCherry, and TetR promoter had no growth. Make 2mL cultures of terminator in LB media - once have growth after 2 hrs with shaking @ 220rpm's, then make 5mL cultures. The 5mL cultures are composed of 4.5mL LB media and 0.5mL from each 2mL cultures. |
| 2. Go to St. Paul: Get BioBrick parts from iGEM notebook - (1)E0030, YFP (2) J06504, RFP (3) R0040, TetR Promoter (4) I732077, GFP + LVA (5) E0032, YFP+LVA and (6) I732078, RBS+RFP+LVA+Term. |
| 3. Order the following: (1) Dual promoter tailed primers to remove RBS, and (2) SSRA tag + Restriction sites tailed primers for GFP and YFP/RFP. |
| 4. Biomedical Genomics Center: Contact them for sequencing problems to target the issue. |
