"
Team:Paris/Notebook/Freezer
From 2008.igem.org
(Difference between revisions)
(→Plasmids: MiniPrep Products) |
|||
| Line 50: | Line 50: | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|width=5%| Number | |width=5%| Number | ||
| - | |width= | + | |width=10%| Name |
| - | |width= | + | |width=60%| Description |
| - | |width= | + | |width=10%| Products in use |
| - | |width= | + | |width=15%| Date |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP1 | | style="background: #ccffcc;" |MP1 | ||
| - | | | + | |[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] |
|Strong constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | |Strong constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | ||
| | | | ||
| Line 62: | Line 62: | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP2 | | style="background: #ccffcc;" |MP2 | ||
| - | | | + | |[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] |
|Medium constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | |Medium constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | ||
|MP2.2 | |MP2.2 | ||
| Line 68: | Line 68: | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP3 | | style="background: #ccffcc;" |MP3 | ||
| - | | | + | |[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] |
|Double terminator (clones 1 + 2) [[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | |Double terminator (clones 1 + 2) [[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | ||
|MP3.3, MP3.4 | |MP3.3, MP3.4 | ||
| Line 74: | Line 74: | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP4 | | style="background: #ccffcc;" |MP4 | ||
| - | | | + | |[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] |
|AraC pBAD (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | |AraC pBAD (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | ||
| | | | ||
| Line 80: | Line 80: | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP5 | | style="background: #ccffcc;" |MP5 | ||
| - | | | + | |[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] |
|Strong RBS (clones 1 + 2) [[Image:Part_icon_rbs.png]] | |Strong RBS (clones 1 + 2) [[Image:Part_icon_rbs.png]] | ||
| | | | ||
| Line 86: | Line 86: | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP6 | | style="background: #ccffcc;" |MP6 | ||
| - | | | + | |[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] |
|ECFP (RBS+LVA+Term) (clones 1 + 2) [[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]] | |ECFP (RBS+LVA+Term) (clones 1 + 2) [[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]] | ||
|MP6'.1, MP6'.2 | |MP6'.1, MP6'.2 | ||
| Line 92: | Line 92: | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP7 | | style="background: #ccffcc;" |MP7 | ||
| - | | | + | |[http://partsregistry.org/Part:BBa_E0241 E0241] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] |
|PoPs to GFP converter (clones 1 + 2) [[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]] | |PoPs to GFP converter (clones 1 + 2) [[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]] | ||
|MP7'.1 | |MP7'.1 | ||
| Line 98: | Line 98: | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP8 | | style="background: #ccffcc;" |MP8 | ||
| - | | | + | |[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] |
|gfp tri-part; strong rbs (clones 1 + 2) [[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]] | |gfp tri-part; strong rbs (clones 1 + 2) [[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]] | ||
| | | | ||
| [[Paris/July 18| July 18, 2007]] | | [[Paris/July 18| July 18, 2007]] | ||
|} | |} | ||
Revision as of 13:26, 22 July 2008
PCR products
| PCR products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | []ng/µL | Date |
| P3 | DGAT-1 | 27 ForDGAT1 | 13 DPst1-DGAT-R | Miniprep pKS::DGAT | 1087 | July 31 | |||
| P4 | DGAT-2 | 12 DPst1-DGAT | 27 Rev-DGAT1 | Miniprep pKS::DGAT | 396 | July 31 | |||
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| MP1 | [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] | Strong constitutive promoter in J61002 (clones 1 + 2) 
| July 15, 2007; Aug. 17, 2007 | |
| MP2 | [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] | Medium constitutive promoter in J61002 (clones 1 + 2)
| MP2.2 | July 15, 2007; Aug. 17, 2007 |
| MP3 | [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] | Double terminator (clones 1 + 2) 
| MP3.3, MP3.4 | August 14, 2007 |
| MP4 | [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] | AraC pBAD (clones 1 + 2)
| July 16, 2007 | |
| MP5 | [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | Strong RBS (clones 1 + 2) 
| July 18, 2007 | |
| MP6 | [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | ECFP (RBS+LVA+Term) (clones 1 + 2) 
| MP6'.1, MP6'.2 | July 18, 2007; July 24, 2007 |
| MP7 | [http://partsregistry.org/Part:BBa_E0241 E0241] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | PoPs to GFP converter (clones 1 + 2)
| MP7'.1 | July 18, 2007; July 24, 2007 |
| MP8 | [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | gfp tri-part; strong rbs (clones 1 + 2)
| July 18, 2007 | |
