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21 May 2008
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Things to do:<br> | Things to do:<br> | ||
| - | Mini-preps of O/N's<br> | + | <strike>Mini-preps of O/N's</strike><br> |
Sequence gel purified fragments from yesterday<br> | Sequence gel purified fragments from yesterday<br> | ||
Transform I0500<br> | Transform I0500<br> | ||
| - | Digest J61003 with Xba/Pst to insert ORF's<br> | + | <strike>Digest J61003 with Xba/Pst to insert ORF's</strike><br> |
| - | Digest minipreps with Xba/Pst to insert into J61003<br> | + | <strike>Digest minipreps with Xba/Pst to insert into J61003</strike><br> |
Run gel of digests and excise correct fragments<br> | Run gel of digests and excise correct fragments<br> | ||
Ligate vector/inserts<br> | Ligate vector/inserts<br> | ||
Revision as of 22:50, 21 May 2008
Volunteers Scheduled for Today
5-8 AS
6-8 DL
Things to do:
Mini-preps of O/N's
Sequence gel purified fragments from yesterday
Transform I0500
Digest J61003 with Xba/Pst to insert ORF's
Digest minipreps with Xba/Pst to insert into J61003
Run gel of digests and excise correct fragments
Ligate vector/inserts
Transform ligation reactions
Lab clean up
Checked all O/N's set up by SZ and WX - all grew except 023 WX. Will set up again tonight.
Followed standard Qiagen miniprep protocol for all o/n's.
The Good and Bad news
The Good News
Our system will work in E.coli
The Bad News
In 2004 Texas submitted the cyanbacterial equivalent of our system, so... Our biobricks would be unique but they would do the exact same thing.
The Good News
I looked up a very promising pathway that detects blue light instead
The Bad News
I can only find a summary of the paper... Here is the link to it
http://www.arabidopsis.org/servlets/TairObject?type=publication&id=501724582
Lab Tip of the Day
When using the NanoVu to measure your DNA concentration, please ensure that you press the measure button and not the calibration button. Also, remember that the instructions for using the NanoVu are posted above the NanoVu. Please refer to these instructions in times of doubt instead of getting Chris to do it for you.
PS: Falafel does not a good pizza make.
