Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→Plasmids: MiniPrep Products)
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|align="center"|2007
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|== Digestion Products ==
 +
<br>
 +
<font color="#FF99FF" size="4">PURPLE = Vector </font><br>
 +
<font color="#ccffcc" size="4">GREEN = Insert </font><br>
 +
{|
 +
|- style="background: #ccccff;"
 +
! colspan="5" style="background: #ccccff;" | Digestion Products
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| Number
 +
|width=20%| Product Name
 +
|width=20%| Matrix Name
 +
|width=5%| Enzyme 1
 +
|width=5%| Enzyme 2
 +
|width=5%| Size
 +
|width=30%| Description
 +
|width=5%| Product Left
 +
|width=5%| []ng/µL
 +
|width=5%| Date
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D1
 +
|<bbpart>pJ23100</bbpart>
 +
|<bbpart>pJ23100</bbpart>
 +
|Spe1
 +
|Pst1
 +
|2096
 +
|Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
 +
|Ø (24/09/07)
 +
|34
 +
|[[Paris/July 17| July 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D2
 +
|<bbpart>mRFP dblTer</bbpart>
 +
|<bbpart>pJ23100</bbpart>
 +
|Spe1
 +
|Pst1
 +
|887
 +
|
 +
|
 +
|27
 +
|[[Paris/July 17| July 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D3
 +
|<bbpart>pJ23107</bbpart>
 +
|<bbpart>pJ23107</bbpart>
 +
|Spe1
 +
|Pst1
 +
|2096
 +
|Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
 +
|Ø (24/09/07)
 +
|32
 +
|[[Paris/July 17| July 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D4
 +
|<bbpart>mRFP dblTer</bbpart>
 +
|<bbpart>pJ23107</bbpart>
 +
|Spe1
 +
|Pst1
 +
|887
 +
|
 +
|
 +
|25
 +
|[[Paris/July 17| July 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D5
 +
|<bbpart>pJ23100</bbpart>
 +
|<bbpart>pJ23100</bbpart>
 +
|EcoR1
 +
|Spe1
 +
|2925
 +
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
 +
|
 +
|28&29
 +
|[[Paris/July 17| July 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D6
 +
|<bbpart>pJ23107</bbpart>
 +
|<bbpart>pJ23107</bbpart>
 +
|EcoR1
 +
|Spe1
 +
|2925
 +
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
 +
|
 +
|31&27
 +
|[[Paris/July 17| July 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D7
 +
|Lox66 DapA E.coli
 +
|Lox66 DapA E.coli (PCR product)
 +
|EcoR1
 +
|Pst1
 +
|977
 +
|
 +
|
 +
|24
 +
|[[Paris/July 17| July 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D8
 +
|Lox66 DapA E.coli
 +
|Lox66 DapA E.coli (PCR product)
 +
|Xba1
 +
|Pst1
 +
|962
 +
|
 +
|
 +
|44
 +
|[[Paris/July 17| July 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D9
 +
|<bbpart>pJ23100</bbpart> ES vector
 +
|<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
 +
|EcoI
 +
|SpeI
 +
|2925
 +
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
 +
|
 +
|80&43
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D10
 +
|<bbpart>pJ23100</bbpart> promoter fw-insert
 +
|<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
 +
|EcoI
 +
|SpeI
 +
|58
 +
|J23100 promoter ready for use as a fw-insert
 +
|
 +
|35&28
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D11
 +
|<bbpart>pJ23107</bbpart> ES vector
 +
|<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
 +
|EcoRI
 +
|SpeI
 +
|2925
 +
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
 +
|
 +
|49,35,28&41
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D12
 +
|<bbpart>pJ23107</bbpart> promoter fw-insert
 +
|<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
 +
|EcoRI
 +
|SpeI
 +
|58
 +
|J23107 promoter ready for usage as a fw-insert
 +
|
 +
|23,25,32&32
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D13
 +
|<bbpart>mRFP</bbpart>
 +
|<bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4)
 +
|SpeI
 +
|PstI
 +
|887
 +
|mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
 +
|
 +
|21&41
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D14
 +
|Cre ORF
 +
|<bbpart>J61047</bbpart> MP9.1 & MP9.2
 +
|XbaI
 +
|PstI
 +
|1063
 +
|Cre ORF extracted for usage as a bw-insert
 +
|Ø D14.1
 +
|38&41
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D15
 +
|<bbpart>B0030</bbpart> bw-vector
 +
|<bbpart>B0030</bbpart> MP5.1 & MP5.2
 +
|SpeI
 +
|PstI
 +
|2076
 +
|bacward vector for cloning a CDS bw-insert after the B0030 RBS
 +
|
 +
|87&42
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D18
 +
|BB dig. lox66DapAE.coli
 +
|lox66-DapAE.coli P5 PCR product
 +
|EcoRI
 +
|PstI
 +
|~960
 +
|PCR product digested for cloning as a BioBrick
 +
|Ø
 +
|44
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D19
 +
|BB dig. lox66DapAsubtilis
 +
|lox66-DapAsubtilis P6 PCR product
 +
|EcoRI
 +
|PstI
 +
|~960
 +
|PCR product digested for cloning as a BioBrick
 +
|Ø
 +
|
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D19'
 +
|BB dig. lox66DapAsubtilis
 +
|lox66-DapAsubtilis P6' PCR product
 +
|EcoRI
 +
|PstI
 +
|~960
 +
|PCR product digested for cloning as a BioBrick
 +
|
 +
|16
 +
|[[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D20
 +
|bw-insert lox66-DapAE.coli
 +
|lox66-DapAE.coli P5 PCR product
 +
|XbaI
 +
|PstI
 +
|~960
 +
|PCR product digested for usage as a backward insert
 +
|Ø
 +
|
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D21
 +
|bw-insert lox66-DapAsubtilis
 +
|lox66-DapAsubtilis P6 PCR product
 +
|XbaI
 +
|PstI
 +
|~960
 +
|PCR product digested for usage as a backward insert
 +
|Ø
 +
|
 +
|[[Paris/July 21| July 21]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D21'
 +
|bw-insert lox66-DapAsubtilis
 +
|lox66-DapAsubtilis P6' PCR product
 +
|XbaI
 +
|PstI
 +
|~960
 +
|PCR product digested for cloning as a BI
 +
|
 +
|34
 +
|[[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D22
 +
|pSB1A2 open vector
 +
|<bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)
 +
|EcoI
 +
|PstI
 +
|Ø D22.1
 +
|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
 +
|Ø (24/09/07)
 +
|D22.2:59
 +
|[[Paris/July 23| July 23]],[[Paris/August 13 | Aug. 13 ]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D23
 +
|AraC/pBad promoter fw-insert
 +
|<bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)
 +
|EcoI
 +
|SpeI
 +
|1233
 +
|AraC/pBad promoter ready for use as a forward insert
 +
|Ø D23.1
 +
|93&53
 +
|[[Paris/July 23| July 23]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D24
 +
|eCFP bw-insert
 +
|<bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)
 +
|XbaI
 +
|PstI
 +
|
 +
|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
 +
|
 +
|56&55
 +
|[[Paris/July 23| July 23]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D26
 +
|gfp-tripart
 +
|<bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)
 +
|XbaI
 +
|PstI
 +
|
 +
|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
 +
|
 +
|47&47
 +
|[[Paris/July 23| July 23]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D27
 +
|lox71-ftsZ bw-insert
 +
|lox71-ftsZ PCR product P7
 +
|XbaI
 +
|PstI
 +
|
 +
|lox71-ftsZ(unmutated) ready for use as a backward insert
 +
|Ø
 +
|
 +
|[[Paris/July 23| July 23]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D27'
 +
|lox71-ftsZ bw-insert
 +
|lox71-ftsZ PCR product P7
 +
|XbaI
 +
|PstI
 +
|
 +
|lox71-ftsZ(unmutated) ready for use as a backward insert
 +
|
 +
|24
 +
|[[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D28
 +
|RBS-DapAColi BB
 +
|P8 (RBS-DapAColi)
 +
|EcoRI
 +
|PstI
 +
|~950
 +
|Biobrick
 +
|
 +
|54
 +
|[[Paris/July 25| July 25]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D29
 +
|RBS-DapAColi
 +
|P8 (RBS-DapAColi)
 +
|XbaI
 +
|PstI
 +
|~950
 +
|Backward insert
 +
|
 +
|44
 +
|[[Paris/July 25| July 25]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D30
 +
|RBS-DapASubtilis BB
 +
|P9 (RBS-DapASubtilis)
 +
|EcoRI
 +
|PstI
 +
|~950
 +
|Biobrick
 +
|
 +
|72
 +
|[[Paris/July 25| July 25]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D31
 +
|RBS-DapASubtilis
 +
|P9 (RBS-DapASubtilis)
 +
|XbaI
 +
|PstI
 +
|~950
 +
|Backward insert
 +
|
 +
|51
 +
|[[Paris/July 25| July 25]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D32
 +
|Lox71-FtsA-FtsZ
 +
|P10 Lox71-FtsA-FtsZ
 +
|XbaI
 +
|PstI
 +
|~2600
 +
|Backward insert - EcoRI site still presents : we can't use EcoRI
 +
|
 +
|47
 +
|[[Paris/July 25| July 25]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D33
 +
|RBS(B0030)CreORF
 +
|L6' (clone 1 & 2)
 +
|XbaI
 +
|PstI
 +
|
 +
|RBS-Cre digestion for use as a BI
 +
|
 +
|29&27
 +
|[[Paris/July 30| July 30]],[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D34
 +
|lox66
 +
|L9' lox66
 +
|SpeI
 +
|PstI
 +
|
 +
|Biobrick plasmid carrying lox66 digested as a BV
 +
|
 +
|18
 +
|[[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D35
 +
|lox71
 +
|L10' lox71
 +
|SpeI
 +
|PstI
 +
|
 +
|Biobrick plasmid carrying lox71 digested as a BV
 +
|
 +
|
 +
|[[Paris/July 30| July 30]],[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D36
 +
|lox71-KmR-lox66
 +
|P11 lox71-KmR-lox66
 +
|EcoRI
 +
|PstI
 +
|
 +
|lox71-KmR-lox66 digested as a biobrick insert
 +
|
 +
|
 +
|[[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D37
 +
|lox71-gfp(tripart) BI
 +
|L12.1 & L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction)
 +
|XbaI
 +
|PstI
 +
|
 +
|lox71_RBS-gfp-double terminator extracted as a backward insert
 +
|
 +
|
 +
|[[Paris/August 6 | Aug. 6 ]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D38
 +
|BB-insert lox66-DapAE.coli
 +
|lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
 +
|EcoRI
 +
|PstI
 +
|
 +
|lox66-dapAE.coli extracted from pTOPO plasmid as a BB insert
 +
|
 +
|
 +
|[[Paris/August 6| Aug. 6]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D39'
 +
|BI-insert lox66-DapAE.coli
 +
|lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
 +
|XbaI
 +
|PstI
 +
|
 +
|lox66-dapAcoli extracted for usage as a backward insert
 +
|
 +
|
 +
|[[Paris/August 7 | Aug. 7 ]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D40
 +
|BB-insert lox71-KmR-lox66
 +
|L25.1 miniprep product (from ligation rxn of [[Paris/July 30| 30/07]]
 +
|EcoRI
 +
|PstI
 +
|
 +
|lox71-KmR-lox66 construct extracted for clonong as a biobrick
 +
|
 +
|
 +
|[[Paris/August 6 | Aug. 6 ]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D41
 +
|araC/pBad-J61002 plasmid SP dig.
 +
|L1.2, L1".1 & L1".2 miniprep product
 +
|SpeI
 +
|PstI
 +
|
 +
|J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS
 +
|
 +
|
 +
|[[Paris/August 13 | Aug. 13 ]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D42
 +
|Lox66 FV
 +
|L9
 +
|EcoRI
 +
|XbaI
 +
|
 +
|pSB1A2-lox66 as a Forward vector
 +
|
 +
|
 +
|[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D43
 +
|gfp-tripart FI
 +
|MP8.1
 +
|EcoRI
 +
|SpeI
 +
|
 +
|gfp-tri part (strongRBS-ORF-T) ready for use as a forward insert
 +
|
 +
|
 +
|[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D44
 +
|<bbpart>B0015</bbpart> terminator BI
 +
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
 +
|XbaI
 +
|Pst1
 +
|
 +
|terminator B0015 extracted for use as a BI
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D45
 +
|<bbpart>B0015</bbpart> terminator FI
 +
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.4 Aug.14)
 +
|EcoRI
 +
|SpeI
 +
|
 +
|terminator B0015 extracted for use as a FI
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D46
 +
|<bbpart>B0015</bbpart> BV
 +
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
 +
|SpeI
 +
|Pst1
 +
|
 +
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> plasmid extracted for use as a BV
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D47
 +
|<bbpart>pSB1A2</bbpart> XP dig.
 +
|<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.1)
 +
|XbaI
 +
|PstI
 +
|
 +
|<bbpart>pSB1A2</bbpart> open vector ready for cloning of BI
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D48
 +
|<bbpart>pSB1A2</bbpart> ES dig.
 +
|<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.2)
 +
|EcoRI
 +
|SpeI
 +
|
 +
|<bbpart>pSB1A2</bbpart> open vector ready for cloning of FI
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D49
 +
|lox71-gfptripart BI
 +
|lox71-gfptripart in <bbpart>pSB1A2</bbpart> (L12.2)
 +
|XbaI
 +
|Pst1
 +
|
 +
|lox71-gfptripart extracted for use as a BI
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D50
 +
|<bbpart>BBa_J61002</bbpart> pTet SP dig.
 +
|<bbpart>BBa_J61002</bbpart> (MP13.1 [[Paris/August 14| Aug. 14]])
 +
|SpeI
 +
|PstI
 +
|
 +
|<bbpart>BBa_J61002</bbpart> pTet promoter vector ready for insertion of CDS as a BI
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D51
 +
|RBS-dapAcoli BBinsert
 +
|pTOPO of RBS-dapAcoli (MPT4.3)
 +
|EcoRI
 +
|Pst1
 +
|
 +
|RBS-dapAcoli extracted for cloning as a biobrick
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D52
 +
|RBS-dapAcoli BI
 +
|pTOPO of RBS-dapAcoli (MPT4.3)
 +
|XbaI
 +
|Pst1
 +
|
 +
|RBS-dapAcoli extracted for use as a BI
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D53
 +
|RBS-dapAsubtilis BBinsert
 +
|pTOPO of RBS-dapAsubtilis (MPT5.1)
 +
|EcoRI
 +
|Pst1
 +
|
 +
|RBS-dapAsubtilis extracted for cloning as a biobrick
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D54
 +
|RBS-dapAsubtilis BI
 +
|pTOPO of RBS-dapAsubtilis (MPT5.1)
 +
|XbaI
 +
|Pst1
 +
|
 +
|RBS-dapAsubtilis extracted for use as a BI
 +
|Ø (24/09/07)
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D55
 +
|RBS-dapAsubtilis FI
 +
|pTOPO of RBS-dapAsubtilis (MPT5.2)
 +
|EcoRI
 +
|SpeI
 +
|
 +
|RBS-dapAsubtilis extracted for use as a FI
 +
|
 +
|
 +
|[[Paris/August 16| August 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D57
 +
|lox71-B0015(T) BI
 +
|(L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart>
 +
|XbaI
 +
|Pst1
 +
|
 +
|lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart>
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D58
 +
|B0015(T)-lox66 BV
 +
|(L32.1, L32.2) B0015(T)-lox66 BV
 +
|SpeI
 +
|PstI
 +
|
 +
|B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart>
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D59
 +
|pTet>>lox71-gfptripart BI
 +
|(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 +
|XbaI
 +
|PstI
 +
|
 +
|pTet>>lox71-gfptripart for use as a BI
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;"  |D61
 +
|lox66-mRFP FV
 +
|(L36.1, L36.2) lox66-mRFP
 +
|EcoRI
 +
|XbaI
 +
|
 +
|lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D62
 +
|lox66-RBSDapAcoli BI
 +
|(L37.1, L37.2) lox66-RBSDapAcoli
 +
|XbaI
 +
|PstI
 +
|
 +
|lox66-RBSDapAcoli ready for use as a Backward insert
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D63
 +
|RBSDapAcoli BI
 +
|(L39.1, L39.2) RBSDapAcoli
 +
|XbaI
 +
|PstI
 +
|
 +
|RBSDapAcoli BI (for insertion behind T-lox66)
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D64
 +
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV
 +
|(MP14.1) <bbpart>BBa_J61025</bbpart>
 +
|SpeI
 +
|PstI
 +
|
 +
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion)
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D65
 +
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV
 +
|(MP14.1) <bbpart>BBa_J61025</bbpart>
 +
|EcoR
 +
|XbaI
 +
|
 +
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion)
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D68
 +
|attB BV
 +
|(MP15.1)
 +
|SpeI
 +
|PstI
 +
|
 +
|vector containing attB for use as a BV
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D69
 +
|gfptripart-lox66 BV
 +
|(L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart>
 +
|SpeI
 +
|PstI
 +
|
 +
|
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D70
 +
|pTet>>lox71-gfptripart FI
 +
|(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 +
|EcoRI
 +
|SpeI
 +
|
 +
|pTet>>lox71-gfptripart for use as a FI
 +
|
 +
|
 +
|[[Paris/September 14| Sep. 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D71
 +
|araC/pBad>>lox71-gfptripart FI
 +
|L35.1 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 +
|EcoRI
 +
|SpeI
 +
|
 +
|araC/pBad>>lox71-gfptripart for use as a FI
 +
|Ø (24/09/07)
 +
|
 +
|[[Paris/September 14| Sep. 14]][[Paris/September 17| Sep. 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D72
 +
|<bbpart>pSB2K3</bbpart> BBvector
 +
|MP16 <bbpart>pSB2K3</bbpart>::attP
 +
|EcoRI
 +
|PstI
 +
|
 +
|Low copy number plasmid, BB vector
 +
|
 +
|
 +
|[[Paris/September 17| Sep. 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #FF99FF;" |D73
 +
|<bbpart>pSB2K3</bbpart> ES open vector
 +
|MP16 <bbpart>pSB2K3</bbpart>::attP
 +
|EcoRI
 +
|SpeI
 +
|
 +
|Comparison of digestion efficiency between Pst and Spe in NEB2
 +
|
 +
|
 +
|[[Paris/September 17| Sep. 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D76
 +
|DGAT BB
 +
|P14 DGAT
 +
|EcoRI
 +
|PstI
 +
|
 +
|DGAT
 +
|Ø (24/09/07)
 +
|
 +
|[[Paris/September 17| Sep. 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D77
 +
|araC/pBad>>lox71-gfptripart BI
 +
|D60 (.1 & .2) digestion product
 +
|FspI
 +
|AhdI
 +
|
 +
|in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
 +
|
 +
|
 +
|[[Paris/September 17| Sep. 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;"  |D78
 +
|araC/pBad>>lox71-gfptripart FI
 +
|D71 (.1 & .2) digestion product
 +
|FspI
 +
|AhdI
 +
|
 +
|in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
 +
|
 +
|
 +
|[[Paris/September 17| Sep. 17]]
|}
|}

Revision as of 15:31, 22 July 2008

PCR products

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date
[[ ]]
[[ ]]

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Products in use Date
MP1 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Strong constitutive promoter in J61002 (clones 1 + 2) Part icon regulatory.png 2007
MP2 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Medium constitutive promoter in J61002 (clones 1 + 2) Part icon regulatory.png MP2.2 2007
MP3 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] Double terminator (clones 1 + 2) Part icon terminator.pngPart icon terminator.png MP3.3, MP3.4 2007
MP4 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] AraC pBAD (clones 1 + 2) Part icon regulatory.png 2007
MP5 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Strong RBS (clones 1 + 2) Part icon rbs.png 2007
MP6 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] ECFP (RBS+LVA+Term) (clones 1 + 2) Part icon rbs.pngPart icon reporter.pngPart icon terminator.png MP6'.1, MP6'.2 2007
MP8 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] gfp tri-part; strong rbs (clones 1 + 2) Part icon rbs.pngPart icon reporter.pngPart icon terminator.png 2007

| | | |== Digestion Products ==
PURPLE = Vector
GREEN = Insert

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description Product Left []ng/µL Date
D1 <bbpart>pJ23100</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 2096 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert Ø (24/09/07) 34 July 17
D2 <bbpart>mRFP dblTer</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 887 27 July 17
D3 <bbpart>pJ23107</bbpart> <bbpart>pJ23107</bbpart> Spe1 Pst1 2096 Vector with the J23107 promoter ready for insertion of a RBS/CDS insert Ø (24/09/07) 32 July 17
D4 <bbpart>mRFP dblTer</bbpart> <bbpart>pJ23107</bbpart> Spe1 Pst1 887 25 July 17
D5 <bbpart>pJ23100</bbpart> <bbpart>pJ23100</bbpart> EcoR1 Spe1 2925 The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. 28&29 July 17
D6 <bbpart>pJ23107</bbpart> <bbpart>pJ23107</bbpart> EcoR1 Spe1 2925 The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. 31&27 July 17
D7 Lox66 DapA E.coli Lox66 DapA E.coli (PCR product) EcoR1 Pst1 977 24 July 17
D8 Lox66 DapA E.coli Lox66 DapA E.coli (PCR product) Xba1 Pst1 962 44 July 17
D9 <bbpart>pJ23100</bbpart> ES vector <bbpart>pJ23100</bbpart> (MP1.1 & MP1.2) EcoI SpeI 2925 Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) 80&43 July 21
D10 <bbpart>pJ23100</bbpart> promoter fw-insert <bbpart>pJ23100</bbpart> (MP1.1 & MP1.2) EcoI SpeI 58 J23100 promoter ready for use as a fw-insert 35&28 July 21
D11 <bbpart>pJ23107</bbpart> ES vector <bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4) EcoRI SpeI 2925 Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter 49,35,28&41 July 21
D12 <bbpart>pJ23107</bbpart> promoter fw-insert <bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4) EcoRI SpeI 58 J23107 promoter ready for usage as a fw-insert 23,25,32&32 July 21
D13 <bbpart>mRFP</bbpart> <bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4) SpeI PstI 887 mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it 21&41 July 21
D14 Cre ORF <bbpart>J61047</bbpart> MP9.1 & MP9.2 XbaI PstI 1063 Cre ORF extracted for usage as a bw-insert Ø D14.1 38&41 July 21
D15 <bbpart>B0030</bbpart> bw-vector <bbpart>B0030</bbpart> MP5.1 & MP5.2 SpeI PstI 2076 bacward vector for cloning a CDS bw-insert after the B0030 RBS 87&42 July 21
D18 BB dig. lox66DapAE.coli lox66-DapAE.coli P5 PCR product EcoRI PstI ~960 PCR product digested for cloning as a BioBrick Ø 44 July 21
D19 BB dig. lox66DapAsubtilis lox66-DapAsubtilis P6 PCR product EcoRI PstI ~960 PCR product digested for cloning as a BioBrick Ø July 21
D19' BB dig. lox66DapAsubtilis lox66-DapAsubtilis P6' PCR product EcoRI PstI ~960 PCR product digested for cloning as a BioBrick 16 July 30
D20 bw-insert lox66-DapAE.coli lox66-DapAE.coli P5 PCR product XbaI PstI ~960 PCR product digested for usage as a backward insert Ø July 21
D21 bw-insert lox66-DapAsubtilis lox66-DapAsubtilis P6 PCR product XbaI PstI ~960 PCR product digested for usage as a backward insert Ø July 21
D21' bw-insert lox66-DapAsubtilis lox66-DapAsubtilis P6' PCR product XbaI PstI ~960 PCR product digested for cloning as a BI 34 July 30
D22 pSB1A2 open vector <bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2) EcoI PstI Ø D22.1 Open pSB1A2 vector for BioBrick EcoRI/PstI insertio Ø (24/09/07) D22.2:59 July 23, Aug. 13
D23 AraC/pBad promoter fw-insert <bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2) EcoI SpeI 1233 AraC/pBad promoter ready for use as a forward insert Ø D23.1 93&53 July 23
D24 eCFP bw-insert <bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2) XbaI PstI eCFP (RBS-OFR_LVA-T) ready for use as a backward insert 56&55 July 23
D26 gfp-tripart <bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2) XbaI PstI gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert 47&47 July 23
D27 lox71-ftsZ bw-insert lox71-ftsZ PCR product P7 XbaI PstI lox71-ftsZ(unmutated) ready for use as a backward insert Ø July 23
D27' lox71-ftsZ bw-insert lox71-ftsZ PCR product P7 XbaI PstI lox71-ftsZ(unmutated) ready for use as a backward insert 24 July 30
D28 RBS-DapAColi BB P8 (RBS-DapAColi) EcoRI PstI ~950 Biobrick 54 July 25
D29 RBS-DapAColi P8 (RBS-DapAColi) XbaI PstI ~950 Backward insert 44 July 25
D30 RBS-DapASubtilis BB P9 (RBS-DapASubtilis) EcoRI PstI ~950 Biobrick 72 July 25
D31 RBS-DapASubtilis P9 (RBS-DapASubtilis) XbaI PstI ~950 Backward insert 51 July 25
D32 Lox71-FtsA-FtsZ P10 Lox71-FtsA-FtsZ XbaI PstI ~2600 Backward insert - EcoRI site still presents : we can't use EcoRI 47 July 25
D33 RBS(B0030)CreORF L6' (clone 1 & 2) XbaI PstI RBS-Cre digestion for use as a BI 29&27 July 30, August 13
D34 lox66 L9' lox66 SpeI PstI Biobrick plasmid carrying lox66 digested as a BV 18 July 30
D35 lox71 L10' lox71 SpeI PstI Biobrick plasmid carrying lox71 digested as a BV July 30, August 13
D36 lox71-KmR-lox66 P11 lox71-KmR-lox66 EcoRI PstI lox71-KmR-lox66 digested as a biobrick insert July 30
D37 lox71-gfp(tripart) BI L12.1 & L12.2 miniprep products (from 30/07 ligation reaction) XbaI PstI lox71_RBS-gfp-double terminator extracted as a backward insert Aug. 6
D38 BB-insert lox66-DapAE.coli lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2) EcoRI PstI lox66-dapAE.coli extracted from pTOPO plasmid as a BB insert Aug. 6
D39' BI-insert lox66-DapAE.coli lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2) XbaI PstI lox66-dapAcoli extracted for usage as a backward insert Aug. 7
D40 BB-insert lox71-KmR-lox66 L25.1 miniprep product (from ligation rxn of 30/07 EcoRI PstI lox71-KmR-lox66 construct extracted for clonong as a biobrick Aug. 6
D41 araC/pBad-J61002 plasmid SP dig. L1.2, L1".1 & L1".2 miniprep product SpeI PstI J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS Aug. 13
D42 Lox66 FV L9 EcoRI XbaI pSB1A2-lox66 as a Forward vector August 13
D43 gfp-tripart FI MP8.1 EcoRI SpeI gfp-tri part (strongRBS-ORF-T) ready for use as a forward insert August 13
D44 <bbpart>B0015</bbpart> terminator BI <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14) XbaI Pst1 terminator B0015 extracted for use as a BI August 16
D45 <bbpart>B0015</bbpart> terminator FI <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.4 Aug.14) EcoRI SpeI terminator B0015 extracted for use as a FI August 16
D46 <bbpart>B0015</bbpart> BV <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14) SpeI Pst1 <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> plasmid extracted for use as a BV August 16
D47 <bbpart>pSB1A2</bbpart> XP dig. <bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.1) XbaI PstI <bbpart>pSB1A2</bbpart> open vector ready for cloning of BI August 16
D48 <bbpart>pSB1A2</bbpart> ES dig. <bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.2) EcoRI SpeI <bbpart>pSB1A2</bbpart> open vector ready for cloning of FI August 16
D49 lox71-gfptripart BI lox71-gfptripart in <bbpart>pSB1A2</bbpart> (L12.2) XbaI Pst1 lox71-gfptripart extracted for use as a BI August 16
D50 <bbpart>BBa_J61002</bbpart> pTet SP dig. <bbpart>BBa_J61002</bbpart> (MP13.1 Aug. 14) SpeI PstI <bbpart>BBa_J61002</bbpart> pTet promoter vector ready for insertion of CDS as a BI August 16
D51 RBS-dapAcoli BBinsert pTOPO of RBS-dapAcoli (MPT4.3) EcoRI Pst1 RBS-dapAcoli extracted for cloning as a biobrick August 16
D52 RBS-dapAcoli BI pTOPO of RBS-dapAcoli (MPT4.3) XbaI Pst1 RBS-dapAcoli extracted for use as a BI August 16
D53 RBS-dapAsubtilis BBinsert pTOPO of RBS-dapAsubtilis (MPT5.1) EcoRI Pst1 RBS-dapAsubtilis extracted for cloning as a biobrick August 16
D54 RBS-dapAsubtilis BI pTOPO of RBS-dapAsubtilis (MPT5.1) XbaI Pst1 RBS-dapAsubtilis extracted for use as a BI Ø (24/09/07) August 16
D55 RBS-dapAsubtilis FI pTOPO of RBS-dapAsubtilis (MPT5.2) EcoRI SpeI RBS-dapAsubtilis extracted for use as a FI August 16
D57 lox71-B0015(T) BI (L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart> XbaI Pst1 lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart> Sep. 14
D58 B0015(T)-lox66 BV (L32.1, L32.2) B0015(T)-lox66 BV SpeI PstI B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart> Sep. 14
D59 pTet>>lox71-gfptripart BI (L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> XbaI PstI pTet>>lox71-gfptripart for use as a BI Sep. 14
D61 lox66-mRFP FV (L36.1, L36.2) lox66-mRFP EcoRI XbaI lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector Sep. 14
D62 lox66-RBSDapAcoli BI (L37.1, L37.2) lox66-RBSDapAcoli XbaI PstI lox66-RBSDapAcoli ready for use as a Backward insert Sep. 14
D63 RBSDapAcoli BI (L39.1, L39.2) RBSDapAcoli XbaI PstI RBSDapAcoli BI (for insertion behind T-lox66) Sep. 14
D64 FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV (MP14.1) <bbpart>BBa_J61025</bbpart> SpeI PstI FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion) Sep. 14
D65 FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV (MP14.1) <bbpart>BBa_J61025</bbpart> EcoR XbaI FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion) Sep. 14
D68 attB BV (MP15.1) SpeI PstI vector containing attB for use as a BV Sep. 14
D69 gfptripart-lox66 BV (L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart> SpeI PstI Sep. 14
D70 pTet>>lox71-gfptripart FI (L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> EcoRI SpeI pTet>>lox71-gfptripart for use as a FI Sep. 14
D71 araC/pBad>>lox71-gfptripart FI L35.1 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> EcoRI SpeI araC/pBad>>lox71-gfptripart for use as a FI Ø (24/09/07) Sep. 14 Sep. 17
D72 <bbpart>pSB2K3</bbpart> BBvector MP16 <bbpart>pSB2K3</bbpart>::attP EcoRI PstI Low copy number plasmid, BB vector Sep. 17
D73 <bbpart>pSB2K3</bbpart> ES open vector MP16 <bbpart>pSB2K3</bbpart>::attP EcoRI SpeI Comparison of digestion efficiency between Pst and Spe in NEB2 Sep. 17
D76 DGAT BB P14 DGAT EcoRI PstI DGAT Ø (24/09/07) Sep. 17
D77 araC/pBad>>lox71-gfptripart BI D60 (.1 & .2) digestion product FspI AhdI in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion Sep. 17
D78 araC/pBad>>lox71-gfptripart FI D71 (.1 & .2) digestion product FspI AhdI in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion Sep. 17
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