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21 May 2008
From 2008.igem.org
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<li>Digested minipreps of last year's genes using Xba and Pst | <li>Digested minipreps of last year's genes using Xba and Pst | ||
<li>Began sequencing the o/n minipreps</ul> | <li>Began sequencing the o/n minipreps</ul> | ||
| + | |||
| + | <u>'''Chris'''</u>: | ||
| + | Got the results back from the thiolase gene sequencing. Chromatograms look good; unfortunately the sequence doesnt seem to be correct. I BLASTed the sequences and came up with Acetyl-CoA Acetyltransferase. Very odd indeed. | ||
==The Good and Bad news== | ==The Good and Bad news== | ||
Revision as of 23:57, 21 May 2008
Volunteers Scheduled for Today
5-8 AS
6-8 DL
Things to do:
Mini-preps of O/N's
Sequence gel purified fragments from yesterday
Transform I0500
Digest J61003 with Xba/Pst to insert ORF's
Digest minipreps with Xba/Pst to insert into J61003
Run gel of digests and excise correct fragments
Ligate vector/inserts
Transform ligation reactions
Lab clean up
Jason:
- Started investigating the Cry1 blue light detection pathway
- Started to build the LUX operon plant biobrick
- Went to Dougs awesome Modeling Course
James:
- Checked all O/N's set up by SZ and WX - all grew except 023 WX. Will set up again tonight.
- Followed standard Qiagen miniprep protocol for all o/n's.
Winnie and Saima:
- Digested J61003 using Xba and Pst
- Digested minipreps of last year's genes using Xba and Pst
- Began sequencing the o/n minipreps
Chris: Got the results back from the thiolase gene sequencing. Chromatograms look good; unfortunately the sequence doesnt seem to be correct. I BLASTed the sequences and came up with Acetyl-CoA Acetyltransferase. Very odd indeed.
The Good and Bad news
The Good News
Our system will work in E.coli
The Bad News
In 2004 Texas submitted the cyanbacterial equivalent of our system, so... Our biobricks would be unique but they would do the exact same thing.
The Good News
I looked up a very promising pathway that detects blue light instead
The Bad News
I can only find a summary of the paper... Here is the link to it
http://www.arabidopsis.org/servlets/TairObject?type=publication&id=501724582
Lab Tip of the Day
When using the NanoVu to measure your DNA concentration, please ensure that you press the measure button and not the calibration button. Also, remember that the instructions for using the NanoVu are posted above the NanoVu. Please refer to these instructions in times of doubt instead of getting Chris to do it for you.
PS: Falafel does not a good pizza make.
For your Consideration
Sound Emitting Bacteria http://www.jstage.jst.go.jp/article/jgam/44/1/44_49/_article
