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Team:The University of Alberta/22 July 2008
From 2008.igem.org
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Can anyone commit to designing these primers and synthetic constructs (ASAP?) | Can anyone commit to designing these primers and synthetic constructs (ASAP?) | ||
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| + | * Did minipreps on O/N's of I0500 (new) col 1 and col 2. They are kept in Miniprep box 2 in freezer. | ||
Revision as of 17:32, 22 July 2008
Mike added links to two protein complementation papers in the Project/Journals section of the Wiki. One of these uses a Vibrio transcription factor (ToxR) fragment and Vibrio promoter in E. coli, and the other uses B-galactosidase in E. coli. Can anyone figure out how to make the appropriate BioBricks to fuse these to the existing ER LBD BioBrick? We should be able to amplify the B-galactosidase fragments from E. coli, but we will need to synthesize the Vibrio fragments and promoter. We will also need to order a lacZ deletion strain of E. coli (listed in the paper) if we don't already have something like it.
Can anyone commit to designing these primers and synthetic constructs (ASAP?)
- Did minipreps on O/N's of I0500 (new) col 1 and col 2. They are kept in Miniprep box 2 in freezer.
