From 2008.igem.org
(Difference between revisions)
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| |align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101] | | |align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101] |
| |align="center"|[[Image:Part_icon_regulatory.png]] | | |align="center"|[[Image:Part_icon_regulatory.png]] |
- | |align="center"|constitutive promoter member family | + | |align="center"|Strongest Constitutive promoter |
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| |align="center"|2008 | | |align="center"|2008 |
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| |align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109] | | |align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109] |
| |align="center"|[[Image:Part_icon_regulatory.png]] | | |align="center"|[[Image:Part_icon_regulatory.png]] |
- | |align="center"|constitutive promoter member family | + | |align="center"|Weak Constitutive promoter |
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| |align="center"|2008 | | |align="center"|2008 |
Revision as of 18:02, 22 July 2008
Paris
PCR products
PCR products
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Number
| Name
| Oligo F
| Oligo R
| Matrix
| Length
| Comments
| Product Left ?
| []ng/µL
| Date
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| [[ ]]
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| [[ ]]
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Plasmids: MiniPrep Products
Plasmids: MiniPrep Products
|
Number
| Name
| Biobricks
| Description
| Products in use
| Date
|
MP1
| [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002]
|
| Strong constitutive promoter in J61002 (clones 1 + 2)
|
| 2007
|
MP2
| [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002]
|
| Medium constitutive promoter in J61002 (clones 1 + 2)
| MP2.2
| 2007
|
MP3
| [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3]
|
| Double terminator (clones 1 + 2)
| MP3.3, MP3.4
| 2007
|
MP4
| [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]
|
| AraC pBAD (clones 1 + 2)
|
| 2007
|
MP5
| [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
|
| Strong RBS (clones 1 + 2)
|
| 2007
|
MP6
| [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
|
| ECFP (RBS+LVA+Term) (clones 1 + 2)
| MP6'.1, MP6'.2
| 2007
|
MP8
| [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
|
| gfp tri-part; strong rbs (clones 1 + 2)
|
| 2007
|
Plasmids: MiniPrep "in preparation"
|
Number
| Name
| Biobricks
| Description
| Products in use
| Date
|
| [http://partsregistry.org/Part:BBa_B0034 B0034]
|
| Strongest RBS
|
| 2008
|
| [http://partsregistry.org/Part:BBa_C0040 C0040]
|
| tetR repressor
|
| 2008
|
| [http://partsregistry.org/Part:BBa_C0079 C0079]
|
| lasR activator with LVA Tag
|
| 2008
|
| [http://partsregistry.org/Part:BBa_C0179 C0179]
|
| lasR activator
|
| 2007
|
| [http://partsregistry.org/Part:BBa_E0030 E0030]
|
| YFP
|
| 2008
|
| [http://partsregistry.org/Part:BBa_E0040 E0040]
|
| GFP
|
| 2008
|
| [http://partsregistry.org/Part:BBa_E1010 E1010]
|
| mRFP
|
| 2008
|
| [http://partsregistry.org/Part:BBa_J23101 J23101]
|
| Strongest Constitutive promoter
|
| 2008
|
| [http://partsregistry.org/Part:BBa_J23109 J23109]
|
| Weak Constitutive promoter
|
| 2008
|
| [http://partsregistry.org/Part:BBa_R0040 R0040]
|
| tetR repressible promoter
|
| 2008
|
| [http://partsregistry.org/Part:BBa_R0079 R0079]
|
| promoter (lasr & PAI regulated)
|
| 2008
|
| [http://partsregistry.org/Part:BBa_S03154 S03154]
|
| intermediate construction
|
| 2008
|
| [http://partsregistry.org/Part:BBa_S03879 S03879]
|
| intermediate construction
|
| 2008
|
Digestion Products
PURPLE = Vector
GREEN = Insert
Digestion Products
|
Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
| Product Left
| []ng/µL
| Date
|
D1
| <bbpart>pJ23100</bbpart>
| <bbpart>pJ23100</bbpart>
| Spe1
| Pst1
| 2096
| Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
| Ø (24/09/07)
| 34
| July 17
|
D2
| <bbpart>mRFP dblTer</bbpart>
| <bbpart>pJ23100</bbpart>
| Spe1
| Pst1
| 887
|
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| 27
| July 17
|