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Team:Johns Hopkins/Notebook/GROUP 3: Short two way stops
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(New page: == GROUP 3: Short two way stops == Date: 7/21/08 status report by: James Part no.: BBa_K110011 Part Description: Between-bud 27-W FRS2-C LtR Part Location (in build a genome lab...)
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(New page: == GROUP 3: Short two way stops == Date: 7/21/08 status report by: James Part no.: BBa_K110011 Part Description: Between-bud 27-W FRS2-C LtR Part Location (in build a genome lab...)
Newer edit →
Revision as of 01:01, 23 July 2008
GROUP 3: Short two way stops
Date: 7/21/08 status report by: James Part no.: BBa_K110011 Part Description: Between-bud 27-W FRS2-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Yes (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1475) Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Current status of this part: Miniprep digestion yielded no product, PCR; ligation; transformation; and miniprep will be repeated by this Wednesday (07/23/08)
status report by: James Part no.: BBa_K110012 Part Description: Between STE2-W and BST1-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Yes (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1475) Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Current status of this part: Miniprep digestion yielded no product, PCR; ligation; transformation; and miniprep will be repeated by this Wednesday (07/23/08)
status report by: James Part no.: BBa_K110013 Part Description: Between-SWP82-W and EMP47-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; No Cloning of PCR product successful: No Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: The PCR of this part yielded a very large product Current status of this part:
