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Team:Johns Hopkins/Notebook/GROUP 2: MATa Specific-promoters
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(New page: {{JHU}} == GROUP 2: MATa Specific-promoters == Status report by Allison and Nate Part no.: BBa_K110008 Part Description: MFA1 (L+R) Part Location: in a labeled box, second shelf ...) |
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Date: 7/10/08 | Date: 7/10/08 | ||
PCR successful? Yes | PCR successful? Yes | ||
| - | http:// | + | [http://www.jhu.edu/iGEM/Group2:MATaSpecificPromoters/2008-7-22.BioBricks%208%20and%2016.Allison%20Suarez%20and%20Nate%20Sotuyo%20.html BioBricks 8 and 16] |
Cloning of PCR product successful: in progress | Cloning of PCR product successful: in progress | ||
Sequencing of cloned PCR product successful: not done | Sequencing of cloned PCR product successful: not done | ||
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Date: 7/10/08 | Date: 7/10/08 | ||
PCR successful? Yes | PCR successful? Yes | ||
| - | http:// | + | [http://www.jhu.edu/iGEM/Group2:MATaSpecificPromoters/2008-7-22.BioBricks%208%20and%2016.Allison%20Suarez%20and%20Nate%20Sotuyo%20.html BioBricks 8 and 16] |
Cloning of PCR product successful: in progress | Cloning of PCR product successful: in progress | ||
Sequencing of cloned PCR product successful: not done | Sequencing of cloned PCR product successful: not done | ||
Revision as of 01:36, 23 July 2008
GROUP 2: MATa Specific-promoters
Status report by Allison and Nate Part no.: BBa_K110008 Part Description: MFA1 (L+R) Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to front door Date: 7/10/08 PCR successful? Yes [http://www.jhu.edu/iGEM/Group2:MATaSpecificPromoters/2008-7-22.BioBricks%208%20and%2016.Allison%20Suarez%20and%20Nate%20Sotuyo%20.html BioBricks 8 and 16] Cloning of PCR product successful: in progress Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: to be determined Current status of this part: PCR was being troubleshooted, appeared to have good results with regular PCR protocol (not touchdown) in which there was a constant annealing temperature of 55 degrees C - see gel
Status report by Allison and Nate Part no.: BBa_K110016 Part Description: Ste2 (R+L) Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to front door Date: 7/10/08 PCR successful? Yes [http://www.jhu.edu/iGEM/Group2:MATaSpecificPromoters/2008-7-22.BioBricks%208%20and%2016.Allison%20Suarez%20and%20Nate%20Sotuyo%20.html BioBricks 8 and 16] Cloning of PCR product successful: in progress Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: to be determined Current status of this part: Both PCR protocols (touchdown and second PCR with constant annealing temperature) produced product of the correct size. BBa_K110016 was used as a control in the second PCR with BBa_K110008.
Status report by Allison and Nate Part no.: BBa_K110008 Part Description: MFA1 (L+R) Part Location: same as above, plates are at 4 degrees refrigerator near front door Date: 7/14/08 PCR successful? Yes Cloning of PCR product of successful? There were mainly light blue colonies (only a couple white colonies) Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: Ligation Current status of this part: plates are at 4 degrees; another ligation/transformation will be completed soon
Status report by Allison and Nate Part no.: BBa_K110016 Part Description: Ste2 (R+L) Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to front door; plates at 4 degrees Date: 7/14/08 PCR successful? Yes Cloning of PCR product successful: There were many blue colonies (similar to the plate of BB_K110008) Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: Ligation Current status of this part: plates are at 4 degrees; another ligation/transformation will be completed soon
Status report by Allison and Nate Part no.: BBa_K110008 Part Description: MFA1 (L+R) Part Location: same as above, plates are at 4 degrees refrigerator near front door Date: 7/22/08 PCR successful? Yes (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&mode=single&page=2 Cloning of PCR product of successful? Yes (approx 60 white colonies between two plates) Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: Current status of this part: 12 mini preps and the restriction digestion were completed; preparing to send 3 samples to be sequenced (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1478)</b>
Status report by Allison and Nate Part no.: BBa_K110016 Part Description: Ste2 (R+L) Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to front door; plates at 4 degrees Date: 7/22/08 PCR successful? Yes Cloning of PCR product successful: Yes (approx 20 white colonies on one plate) Joining of validated part to adjacent part(s) status: not done Problems to be solved: Current status of this part: 12 mini preps and the restriction digestion were completed; preparing to send 3 samples to be sequenced (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1478)
