Team:IIT Madras

From 2008.igem.org

(Difference between revisions)
Line 7: Line 7:
{|align="left"
{|align="left"
-
|width="100pt"|<strong>IIT Madras</strong>
+
|
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
 
 +
''''Who are we''''
 +
 
 +
IIT Madras team overview
 +
 
|[[Image:Team.png|right|frame|Team picture]]
|[[Image:Team.png|right|frame|Team picture]]
|-
|-
-
|width="100pt"|<strong>Overview</strong>
+
|
-
|Our project looks at a specific category of bacterial transcription factors. Sigma factors are prokaryotic transcription initiation factors that enable the specific binding of RNA polymerase to a variety of promoters. Escherichia coli sigma factors function to differentially express various sets of genes under different environmental conditions.
+
 
 +
''''What are we working on?'''
 +
 
 +
Our project looks at a specific category of bacterial transcription factors, Sigma factors. These are prokaryotic transcription initiation factors that enable the specific binding of RNA polymerase to a variety of promoters. Escherichia coli sigma factors function to differentially express various sets of genes under different environmental conditions.
 +
 
Our goal is to assemble a library of sigma-dependent and lac contorlled promoters, and submit these to the Registry of Standard Biological Parts. This "StressKit" can be used to control gene expression specifically during each phase of the bacterial growth curve and will also give users the ability to regulate gene expression using temperature, pH and other stress inducers as external cues.
Our goal is to assemble a library of sigma-dependent and lac contorlled promoters, and submit these to the Registry of Standard Biological Parts. This "StressKit" can be used to control gene expression specifically during each phase of the bacterial growth curve and will also give users the ability to regulate gene expression using temperature, pH and other stress inducers as external cues.
 +
We've chosen 4 special sigma factors (S,H,F,E) in addition to the houskeeping sigma 70 to include in our library. Our design attempts to engineer the regulation achievable with IPTG fused with the differential expression of the sigma factor dependent promoters.
We've chosen 4 special sigma factors (S,H,F,E) in addition to the houskeeping sigma 70 to include in our library. Our design attempts to engineer the regulation achievable with IPTG fused with the differential expression of the sigma factor dependent promoters.
|[[Image:Team.png|right|frame|Project Overview]]
|[[Image:Team.png|right|frame|Project Overview]]
|}
|}
-
<!--- The Mission, Experiments --->
 

Revision as of 04:50, 23 July 2008

Home About Us Project Details Notebook

'Who are we'

IIT Madras team overview

Team picture

'What are we working on?

Our project looks at a specific category of bacterial transcription factors, Sigma factors. These are prokaryotic transcription initiation factors that enable the specific binding of RNA polymerase to a variety of promoters. Escherichia coli sigma factors function to differentially express various sets of genes under different environmental conditions.

Our goal is to assemble a library of sigma-dependent and lac contorlled promoters, and submit these to the Registry of Standard Biological Parts. This "StressKit" can be used to control gene expression specifically during each phase of the bacterial growth curve and will also give users the ability to regulate gene expression using temperature, pH and other stress inducers as external cues.

We've chosen 4 special sigma factors (S,H,F,E) in addition to the houskeeping sigma 70 to include in our library. Our design attempts to engineer the regulation achievable with IPTG fused with the differential expression of the sigma factor dependent promoters.

Project Overview
Retrieved from "http://2008.igem.org/Team:IIT_Madras"