Team:NTU-Singapore/Notebook/24 July 2008

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(New page: =Thursday 24 July= *RESULT from Wednesday ligation and transformation: **E7-Term got 7 colonies!! **Lysis-standard vector and LsrA-standard vector got lots of colonies!! **Lysis-Term also...)
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Revision as of 08:06, 24 July 2008

Thursday 24 July

  • RESULT from Wednesday ligation and transformation:
    • E7-Term got 7 colonies!!
    • Lysis-standard vector and LsrA-standard vector got lots of colonies!!
    • Lysis-Term also got 5 colonies!!

Hung,Darius:

  • Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.
    RESULT:
    • Digesting for 1 hour and 2 hour didn't make much differences.
  • Inoculation: of the colonies we obtained this morning.