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Team:NTU-Singapore/Notebook/24 July 2008
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(New page: =Thursday 24 July= *RESULT from Wednesday ligation and transformation: **E7-Term got 7 colonies!! **Lysis-standard vector and LsrA-standard vector got lots of colonies!! **Lysis-Term also...) |
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*Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.<br>'''RESULT:''' | *Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.<br>'''RESULT:''' | ||
**Digesting for 1 hour and 2 hour didn't make much differences. | **Digesting for 1 hour and 2 hour didn't make much differences. | ||
| - | ** | + | **All 3 Lysis-Term bands were actually just Term. So all the 3 colonies we obtained just contained the uncut Terminator.<br>'''NOTE:''' |
| + | ***For 2% gel, we should run for at least 1 hour in order to see clear and distinct bands. | ||
| + | ***Terminator vector is 3kb. (as contain both AmpR and KanR), not 2kb as presumed. | ||
| + | ***Bluescript vector is 2.5kb. | ||
*Inoculation: of the colonies we obtained this morning. | *Inoculation: of the colonies we obtained this morning. | ||
Revision as of 08:21, 24 July 2008
Thursday 24 July
- RESULT from Wednesday ligation and transformation:
- E7-Term got 7 colonies!!
- Lysis-standard vector and LsrA-standard vector got lots of colonies!!
- Lysis-Term also got 5 colonies!!
Hung,Darius:
- Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.
RESULT:- Digesting for 1 hour and 2 hour didn't make much differences.
- All 3 Lysis-Term bands were actually just Term. So all the 3 colonies we obtained just contained the uncut Terminator.
NOTE:- For 2% gel, we should run for at least 1 hour in order to see clear and distinct bands.
- Terminator vector is 3kb. (as contain both AmpR and KanR), not 2kb as presumed.
- Bluescript vector is 2.5kb.
- Inoculation: of the colonies we obtained this morning.
