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Team:BCCS-Bristol/Calendar-Notebook/1 July 2008
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| + | <html><link rel="stylesheet" href="http://www.chofski.co.uk/iGEM/bccs-igem.css" type="text/css"></html> | ||
| + | __NOTOC__ | ||
| + | <div class="bccsNavBar"> | ||
| + | {| align="center" | ||
| + | !align="center"|[[Team:BCCS-Bristol|Home]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Team|The Team]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Project|The Project]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Parts|Submitted Parts]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Modeling|Modelling]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Notebook|Wet Lab]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Calendar|Calendar]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Misc|Miscellaneous]] | ||
| + | |} | ||
| + | <br> | ||
| + | </div> | ||
| + | |||
This is a page for any solutions | This is a page for any solutions | ||
Revision as of 13:55, 24 July 2008
This is a page for any solutions
| Media for Plates | 0.3% 400ml | 0.15% 150ml | 0.5% 150ml |
| Bactotryptone | 4g | 1.5g | 1.5g |
| NaCl | 2g | 0.75g | 0.75g |
| BactoAgar | 1.2g | 0.225g | 0.75g |
in addition did a streak out for master colonies of MG1655 in LB from table below overnight at 37oC
| 10ml LB | ' |
| Yeast Extract | 0.05 |
| Tryptone | 0.1 |
| Nacl | 0.1 |
SOC medium in preparation for Biobrick transformation this consisted of
| Trypton | 2g |
| Yeast Extract | 0.5g |
| NaCl | 0.05g |
in 95ml of water, then autoclaved. after this add this:
| MgCl2 | 0.5ml 2M solution |
| Glucose | 2ml 1M |
then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
