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Team:BCCS-Bristol/Calendar-Notebook/15 July 2008
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| + | <html><link rel="stylesheet" href="http://www.chofski.co.uk/iGEM/bccs-igem.css" type="text/css"></html> | ||
| + | __NOTOC__ | ||
| + | <div class="bccsNavBar"> | ||
| + | {| align="center" | ||
| + | !align="center"|[[Team:BCCS-Bristol|Home]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Team|The Team]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Project|The Project]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Parts|Submitted Parts]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Modeling|Modelling]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Notebook|Wet Lab]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Calendar|Calendar]] | ||
| + | !align="center"|[[Team:BCCS-Bristol/Misc|Miscellaneous]] | ||
| + | |} | ||
| + | <br> | ||
| + | </div> | ||
| + | |||
Made SOC medium in preparation for Biobrick transformation this consisted of | Made SOC medium in preparation for Biobrick transformation this consisted of | ||
Revision as of 13:57, 24 July 2008
Made SOC medium in preparation for Biobrick transformation this consisted of
| Trypton | 2g |
| Yeast Extract | 0.5g |
| NaCl | 0.05g |
in 95ml of water, then autoclaved. after this add this:
| MgCl2 | 0.5ml 2M solution |
| Glucose | 2ml 1M |
then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
had a go at making wells as in the diagram (UPLOAD DIAGRAM), needs diff glue
Recorded motility comparison, turns out we messed up and tried to grow MG1655 on streptomycin, therefore we made a repeat of this experiment
