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Minnesota/24 July 2008
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Revision as of 16:08, 24 July 2008
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| 1. Base Vector Plasmid Maxi Prep Base Vector: Grew another culture of different base vector colonies, so now need to plasmid prep the base vector so the DNA/plasmid will be extracted from the rest of the cell. |
| 2. Spectrophotometry: Excellent results from spec'ing new base vector plasmid. Very high concentration of DNA. |
| 3. Base Vector & Promoter Double Digest: 50uL Reaction Mixture. Digest incubation for 2 hours @ 37C. Heat inactivate enzyme for 15 minutes @ 65C in a water bath. Follow the table below for pre-incubation steps: |
