Team:Paris/July 24

From 2008.igem.org

(Difference between revisions)
(→Results of digestions : Electrophoresis)
(→Results of digestions : Electrophoresis)
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|align="center"|'''Enzyme 1'''
|align="center"|'''Enzyme 1'''
|align="center"|'''Enzyme 2'''
|align="center"|'''Enzyme 2'''
 +
|align="center"|'''Observation'''
|align="center"|'''Expected Size Matrix'''
|align="center"|'''Expected Size Matrix'''
|align="center"|'''Expected Size BioBrick'''
|align="center"|'''Expected Size BioBrick'''
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! rowspan="2"| XbaI
! rowspan="2"| XbaI
! rowspan="2"| PstI
! rowspan="2"| PstI
 +
! rowspan="2"| BI
! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
! rowspan="2"| 34 pb
! rowspan="2"| 34 pb
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|-
|-
|align="center"|3
|align="center"|3
-
|align="center"|EcoRI
+
|align="center"| EcoRI
-
|align="center"|XbaI
+
|align="center"| XbaI
 +
|align="center"| FV
|align="center"| 2076 pb
|align="center"| 2076 pb
|align="center"| 15 pb
|align="center"| 15 pb
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|-
|-
|align="center"|4
|align="center"|4
-
|align="center"|SpeI
+
! rowspan="2"| SpeI
-
|align="center"|PstI
+
! rowspan="2"| PstI
 +
! rowspan="2"| BV
 +
! rowspan="2"| 2077 pb
 +
! rowspan="2"| 14 pb
|align="center"|
|align="center"|
|align="center"|
|align="center"|
|-
|-
|align="center"|5
|align="center"|5
-
|align="center"|SpeI
 
-
|align="center"|PstI
 
|align="center"|
|align="center"|
|align="center"|
|align="center"|
|-
|-
-
|align="center"|D101
+
! rowspan="2" style="background: #ccccff;" | D101
-
|align="center"|MP101
+
|align="center"|1
-
|align="center"|J23101
+
! rowspan="2"| SpeI
-
|align="center"|SpeI
+
! rowspan="2"| PstI
-
|align="center"|PstI
+
! rowspan="2"| BV
 +
! rowspan="2"| 2057 pb
 +
! rowspan="2"| 34 pb
 +
|align="center"|
 +
|align="center"|
 +
|-
|align="center"|2
|align="center"|2
 +
|align="center"|
 +
|align="center"|
|-
|-
|align="center"|D102
|align="center"|D102

Revision as of 16:31, 24 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 (=MP1) J23100
MP117 (=MP2) J23107
MP118 (=MP3) B0015
MP119 (=MP4) I0500
MP120 (=MP5) B0030
MP121 (=MP6) E0422
MP122 (=MP8) E0840



Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3


Results of digestions : Electrophoresis

Name Tube N° Enzyme 1 Enzyme 2 Observation Expected Size Matrix Expected Size BioBrick Measured Size Matrix Measured Size BioBrick
D100 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D101 1 SpeI PstI BV 2057 pb 34 pb
2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3
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