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Team:The University of Alberta/24 July 2008
From 2008.igem.org
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*if all goes well the digested product should be B0034+TETR | *if all goes well the digested product should be B0034+TETR | ||
**Column Purified the digested product. | **Column Purified the digested product. | ||
| - | * | + | **Then ligated the B0034+TETR with LacIQERE giving us a complete system 2. |
| + | **PCRed the digested product i.e tetR+BOO34 | ||
*Digested pTet with Spe/Pst. | *Digested pTet with Spe/Pst. | ||
*Digested RFP with Xba/Pst. | *Digested RFP with Xba/Pst. | ||
Revision as of 21:16, 24 July 2008
General
- Did mini-preps of J61003 because we were running out
- Digested BisDA/B with single digests using AgeI and NgoMIV to test the enzymes because weve been getting cruddy digests when using both of them
System 2
Today we have taken the ligation from yesterday and transformed it as well as digested it.
- if all goes well the digested product should be B0034+TETR
- Column Purified the digested product.
- Then ligated the B0034+TETR with LacIQERE giving us a complete system 2.
- PCRed the digested product i.e tetR+BOO34
- Digested pTet with Spe/Pst.
- Digested RFP with Xba/Pst.
System 3
- Digested 5ul of J61003 with Xba/Pst; this will let us insert the Tryp biobrick digested and ligated yesterday
- Ran the digested J6 on a gel; gel purified
