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Template:Team:UC Berkeley/Notebook/SC construction
From 2008.igem.org
(Difference between revisions)
| Line 233: | Line 233: | ||
Construction of {rbs_PhoA>} basic part K112614 | Construction of {rbs_PhoA>} basic part K112614 | ||
| - | PCR | + | PCR sc010/sc013 on MG1655 (112 bp, EcoRI/BamHI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112605 | Product is pBca1256-K112605 | ||
| Line 245: | Line 245: | ||
== pBca1256-K112615 == | == pBca1256-K112615 == | ||
<pre> | <pre> | ||
| - | Construction of {rbs_LamB>} basic part | + | Construction of {rbs_LamB>} basic part K112615 |
| - | PCR | + | PCR sc011/sc014 on MG1655 (126 bp, EcoRI/BamHI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112605 | Product is pBca1256-K112605 | ||
Revision as of 05:24, 25 July 2008
pBca1256-K112600
Construction of {<AP>} basic part K112600
Wobble PCR SC001/SC002 (66 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112600
----------------------------------------------------
SC001F Forward Biobricking of <AP-tag>
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC002R Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112601
Construction of {<AP!} basic part K112601
Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001 Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003 Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112602
Construction of {<FLAG>} basic part K112602
Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112602
---------------------------------------------------
SC004 Forward Biobricking of <FLAG-tag>
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC005 Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc
pBca1256-K112603
Construction of {<FLAG!} basic part K112603
Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112603
-----------------------------------------------------
SC004 Forward Biobricking of <FLAG-tag!
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC006 Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
pBca1256-K112604
Construction of {rbs_barstar>} basic part K112604
PCR mea035/sc007 on Bacillus amyloliquefaciens gen. (320 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112604
-------------------------------
mea035 Fwd biobricking of {rbs_barstar>}
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
sc007 Reverse biobricking of {rbs_barstar>} (Not needed...)
cgttaGGATCCagaaagtatgatggtgatgtcgc
pBca1256-K112403
Construction of {PhoA>} basic part K112403
CR cb10007/cb10008 on MG1655 (95 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112403
-------------------------------------------------------------
cb10007 Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
cb10008 Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112404
Construction of {LamB>} basic part K112404
PCR cb10009/cb10010 on MG1655 (107 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112404
-------------------------------------------------------------
cb10009 Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
cb10010 Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112605
Construction of {a~PhoA>} basic part K112605
PCR sc008/cb10008-phoA-R on MG1655 (100 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc008 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaGtgaaacaaagcactattgcactggc
cb10008 Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112606
Construction of {a~LamB>} basic part K112606
PCR sc009/cb10010-lamB-R on MG1655 (111 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256- K112606
--------------------------------------------------
sc009 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
cb10010 Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112607
Construction of {rbs_PhoA>} basic part K112607
PCR sc010/cb10008-PhoA-R on MG1655 (113 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112607
------------------------------------------------------
sc010 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
cb10008 Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112608
Construction of {rbs_LamB>} basic part K112608
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655 (126 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112608
------------------------------------------------------
sc011 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
cb10010 Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112609
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
PCR sc012/cb10008-phoA-R on MG1655 (100 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008 Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112610
Construction of {PhoA>} basic part K112610
PCR cb10007/sc013 on MG1655 (94 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112610
------------------------------------------------------
cb10007 Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
sc013 Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac
pBca1256-K112611
Construction of {LamB>} basic part K112611
PCR cb10009/sc014 on MG1655 (107 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
cb10009 Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
sc014 Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112612
Construction of {a~PhoA>} basic part K112612
PCR sc012/sc013 on MG1655 (98 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
sc013 Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac
pBca1256-K112613
Construction of {a~LamB>} basic part K112613
PCR sc009/sc014 on MG1655 (111 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc009 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
sc014 Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112614
Construction of {rbs_PhoA>} basic part K112614
PCR sc010/sc013 on MG1655 (112 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc010 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
sc013 Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac
pBca1256-K112615
Construction of {rbs_LamB>} basic part K112615
PCR sc011/sc014 on MG1655 (126 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc011 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
sc014 Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
