"
Team:Paris/Modeling/Programs
From 2008.igem.org
(Difference between revisions)
(→Finding Parameters) |
(→Finding Parameters) |
||
| Line 13: | Line 13: | ||
* (K^n) = "activation constant : dissociation constant of the fixation of x on py" <br> | * (K^n) = "activation constant : dissociation constant of the fixation of x on py" <br> | ||
* n = "stoechiometric coef. of x in the fixation of x on py" <br> | * n = "stoechiometric coef. of x in the fixation of x on py" <br> | ||
| + | |||
| + | After some trials, we have obtained interesting results in terms of precision. We are now trying to test the robustness of the estimations in order to quantify the influence of biological variance in the results as well as the influence of the number of points available. | ||
| + | |||
| + | [[Image:Estimation.jpg]] | ||
Revision as of 08:55, 25 July 2008
Finding Parameters
This program aims at fill this data bank, from experimental data obtained by our wet lab.
We are looking for constants involved in Hill functions :
- Inducer x on py : formation rate of y = b*(x^n/(K^n + x^n))
- Inhibitor x on py : formation rate of y = b*((K^n)/(K^n + x^n))
With following chemical interpretations :
- b = "quantity of DNA multiplied by the transduction rate"
- (K^n) = "activation constant : dissociation constant of the fixation of x on py"
- n = "stoechiometric coef. of x in the fixation of x on py"
After some trials, we have obtained interesting results in terms of precision. We are now trying to test the robustness of the estimations in order to quantify the influence of biological variance in the results as well as the influence of the number of points available.
