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Minnesota/25 July 2008
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|'''1. No growth on plates:''' Experiments on 07-24-08 failed to show growth on Kanamycin resistant plates. | |'''1. No growth on plates:''' Experiments on 07-24-08 failed to show growth on Kanamycin resistant plates. | ||
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| + | |'''2. Redoing ligation:''' Using left over double digest from 07-24-08, But increase the amount of ligase in the reaction and performing the ligations @ 60C which is the optimal temperature for the ligase. | ||
| + | |- | ||
| + | |'''3. Redoing transformations:''' Redo with new ligated products by adding much less DNA/plasmid to the TOP10 Cells, b/c if put too much ligated DNA then the cells do not transform properly (as stated by iGEM troubleshooting). | ||
Revision as of 15:09, 25 July 2008
| Back to Notebook Home | |
| Go to Previous Day (July 24) | Go to Next Day (July 28) |
| 1. No growth on plates: Experiments on 07-24-08 failed to show growth on Kanamycin resistant plates. |
| 2. Redoing ligation: Using left over double digest from 07-24-08, But increase the amount of ligase in the reaction and performing the ligations @ 60C which is the optimal temperature for the ligase. |
| 3. Redoing transformations: Redo with new ligated products by adding much less DNA/plasmid to the TOP10 Cells, b/c if put too much ligated DNA then the cells do not transform properly (as stated by iGEM troubleshooting). |
