User:University of Washington/25 July 2008
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+ | ==LuxR from AraC and TetR== | ||
+ | -Received primers to biobrick Elowitz's plasmid. | ||
+ | |||
+ | -Did PCR amplification on Elowitz's plasmid. | ||
+ | |||
+ | <table> | ||
+ | <tr> | ||
+ | <th>reagents</th><th>amount per reaction(ul)</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>sterile dH2O</td><td>34.5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>buffer for Taq (-MgCl2)</td><td>5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>10 mM dNTPs</td><td>1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>MgCl2</td><td>2</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>F primer(#436)</td><td>2.5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>R primer(#436)</td><td>2.5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Taq</td><td>0.5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>DNA template</td><td>2</td> | ||
+ | </tr> | ||
+ | </table> | ||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] |
Revision as of 20:54, 25 July 2008
LuxR from AraC and TetR
-Received primers to biobrick Elowitz's plasmid.
-Did PCR amplification on Elowitz's plasmid.
reagents | amount per reaction(ul) |
---|---|
sterile dH2O | 34.5 |
buffer for Taq (-MgCl2) | 5 |
10 mM dNTPs | 1 |
MgCl2 | 2 |
F primer(#436) | 2.5 |
R primer(#436) | 2.5 |
Taq | 0.5 |
DNA template | 2 |
Back to Team:University_of_Washington/Notebook#Notebook