User:University of Washington/25 July 2008

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(Difference between revisions)
(→LuxR from AraC and TetR)
(→LuxR from AraC and TetR)
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</tr>
</tr>
<tr>
<tr>
-
   <td>buffer for Taq (-MgCl2)</td><td>5</td>
+
   <td>buffer for Taq (-MgCl2)</td><td align=center>5</td>
</tr>
</tr>
<tr>
<tr>
-
   <td>10 mM dNTPs</td><td>1</td>
+
   <td>10 mM dNTPs</td><td align=center>1</td>
</tr>
</tr>
<tr>
<tr>
-
   <td>MgCl2</td><td>2</td>
+
   <td>MgCl2</td><td align=center>2</td>
</tr>
</tr>
<tr>
<tr>
-
   <td>F primer(#436)</td><td>2.5</td>
+
   <td>F primer(#436)</td><td align=center>2.5</td>
</tr>
</tr>
<tr>
<tr>
-
   <td>R primer(#436)</td><td>2.5</td>
+
   <td>R primer(#436)</td><td align=center>2.5</td>
</tr>
</tr>
<tr>
<tr>
-
   <td>Taq</td><td>0.5</td>
+
   <td>Taq</td><td align=center>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
   <td>DNA template</td><td>2</td>
+
   <td>DNA template</td><td align=center>2</td>
</tr>
</tr>
</table>
</table>

Revision as of 20:55, 25 July 2008

LuxR from AraC and TetR

-Received primers to biobrick Elowitz's plasmid.

-Did PCR amplification on Elowitz's plasmid.

reagentsamount per reaction(ul)
sterile dH2O34.5
buffer for Taq (-MgCl2)5
10 mM dNTPs1
MgCl22
F primer(#436)2.5
R primer(#436)2.5
Taq0.5
DNA template2


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