User:University of Washington/25 July 2008

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(Difference between revisions)
(→LuxR from AraC and TetR)
(→LuxR from AraC and TetR)
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   <td>DNA template</td><td align=center>2</td>
   <td>DNA template</td><td align=center>2</td>
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*Thermocycling
*Thermocycling
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   <th>Segment</th><th>Cycles</th><th>Temperature</th><th>Time</th>
   <th>Segment</th><th>Cycles</th><th>Temperature</th><th>Time</th>

Revision as of 21:40, 25 July 2008

LuxR from AraC and TetR

-Received primers to biobrick Elowitz's plasmid.

-Did PCR amplification on Elowitz's plasmid.

  • Reaction Set-Up
reagentsamount per reaction(ul)
sterile dH2O34.5
buffer for Taq (-MgCl2)5
10 mM dNTPs1
MgCl22
F primer(#436)2.5
R primer(#436)2.5
Taq0.5
DNA template2
  • Thermocycling
SegmentCyclesTemperatureTime
1195°C1 minute
23095°C30 seconds
50°C1 min
72°C1 min
3172°C7 minutes
4-4°Cinfinite

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