Team:Paris/July 27

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(New page: === Results of extraction : Electrophoresis === conditions : * 10µl of ladder 1 kb * 2µl of extraction added with 2µl of loading Dye 6x * migration ~30min at 100W * Gel 1, 2, 3, 4...)
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Results of extraction : Electrophoresis

conditions :

  • 10µl of ladder 1 kb
  • 2µl of extraction added with 2µl of loading Dye 6x
  • migration ~30min at 100W
  • Gel 1, 2, 3, 4, 5 = 0.8%
  • Gel 6 = 1,5%


gel1 gel1 gel2 gel2 gel3 gel3 gel4 gel4


gel5 gel5 gel6 gel6


Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb 1800 pb 40 pb 6 2
2 6 3
3 EcoRI XbaI FV 2076 pb 15 pb 2000 pb - 1 2
4 SpeI PstI BV 2077 pb 14 pb 2000 pb - 1 3
5 1 4
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 700 pb 1 5
2 1 6
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 700 pb 1 7
2 1 8
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb 2000 pb - 1 9
2 1 10
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb 2000 pb - 1 11
2 1 12
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb 3000 pb - 2 5
2 XbaI PstI BI 2057 pb 707 pb 2000 pb 700 pb 2 2
3 2 3
4 EcoRI SpeI FI 2056 pb 708 pb 2000 pb 700 pb 2 4
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb 2700 pb - 2 6
2 XbaI PstI BI 2057 pb 725 pb 2000 pb 700 pb 2 7
3 2 8
4 EcoRI SpeI FI 2756 pb 726 pb 3000 pb 700 pb 2 9
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb 5000 pb 800 pb 2 10
2 XbaI PstI BI 4003 pb 778 pb 5000 pb 800 pb 2 11
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb 2000 pb 800 pb 2 12
2 XbaI PstI BI 4403 pb 745 pb 2000 pb 800 pb 3 2
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb 3000 pb 700 pb 3 3
2 XbaI PstI BI 3167 pb 745 pb 3000 pb 700 pb 3 4
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb 2000 pb 700 pb 3 5
2 XbaI PstI BI 2057 pb 742 pb 2000 pb 700 pb 3 6
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb 5000 pb 700 pb 3 7
2 XbaI PstI BI 4403 pb 703 pb 5000 pb 700 pb 3 8
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 900 pb 3 9
2 3 10
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 900 pb 3 11
2 3 12
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb 3000 pb - 4 2
2 4 3
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb 6000 pb - 4 4
2 4 5
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb 2000 pb 50 pb 6 4
2 6 5
3 EcoRI XbaI FV 2079 pb 15 pb 2200 pb - 4 6
4 SpeI PstI BV 2080 pb 14 pb 2000 pb - 4 7
5 4 8
D121 E0422 1 XbaI PstI BI 2057 pb 939 pb 2000 pb 1100 pb 4 9
2 4 10
3 4 11
D122 E0 1 XbaI PstI BI 2057 pb 900 pb 2000 pb 1000 pb 4 12
2 2000 pb 900 pb 5 2
3 5 3


==> conclusion : all the digestion have succeed.....GREAT !