Team:Paris/July 27
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(New page: === Results of extraction : Electrophoresis === conditions : * 10µl of ladder 1 kb * 2µl of extraction added with 2µl of loading Dye 6x * migration ~30min at 100W * Gel 1, 2, 3, 4...)
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(New page: === Results of extraction : Electrophoresis === conditions : * 10µl of ladder 1 kb * 2µl of extraction added with 2µl of loading Dye 6x * migration ~30min at 100W * Gel 1, 2, 3, 4...)
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Revision as of 14:17, 28 July 2008
Results of extraction : Electrophoresis
conditions :
- 10µl of ladder 1 kb
- 2µl of extraction added with 2µl of loading Dye 6x
- migration ~30min at 100W
- Gel 1, 2, 3, 4, 5 = 0.8%
- Gel 6 = 1,5%
Name | BioBrick | Tube N° | Enz 1 | Enz 2 | Obs | Exp Size Matrix | Exp Size BB | Mea Size Matrix | Mea Size BB | Gel | Band |
D100 | B0034 | 1 | XbaI | PstI | BI | 2057 pb | 34 pb | 1800 pb | 40 pb | 6 | 2 |
---|---|---|---|---|---|---|---|---|---|---|---|
2 | 6 | 3 | |||||||||
3 | EcoRI | XbaI | FV | 2076 pb | 15 pb | 2000 pb | - | 1 | 2 | ||
4 | SpeI | PstI | BV | 2077 pb | 14 pb | 2000 pb | - | 1 | 3 | ||
5 | 1 | 4 | |||||||||
D101 | J23101 | 1 | SpeI | PstI | BV | 2100 pb | 883 pb | 2000 pb | 700 pb | 1 | 5 |
2 | 1 | 6 | |||||||||
D102 | J23109 | 1 | SpeI | PstI | BV | 2100 pb | 883 pb | 2000 pb | 700 pb | 1 | 7 |
2 | 1 | 8 | |||||||||
D103 | R0079 | 1 | SpeI | PstI | BV | 2222 pb | 14 pb | 2000 pb | - | 1 | 9 |
2 | 1 | 10 | |||||||||
D104 | R0040 | 1 | SpeI | PstI | BV | 2119 pb | 14 pb | 2000 pb | - | 1 | 11 |
2 | 1 | 12 | |||||||||
D105 | S03154 | 1 | SpeI | PstI | BV | 2750 pb | 14 pb | 3000 pb | - | 2 | 5 |
2 | XbaI | PstI | BI | 2057 pb | 707 pb | 2000 pb | 700 pb | 2 | 2 | ||
3 | 2 | 3 | |||||||||
4 | EcoRI | SpeI | FI | 2056 pb | 708 pb | 2000 pb | 700 pb | 2 | 4 | ||
D106 | S03879 | 1 | SpeI | PstI | BV | 2768 pb | 14 pb | 2700 pb | - | 2 | 6 |
2 | XbaI | PstI | BI | 2057 pb | 725 pb | 2000 pb | 700 pb | 2 | 7 | ||
3 | 2 | 8 | |||||||||
4 | EcoRI | SpeI | FI | 2756 pb | 726 pb | 3000 pb | 700 pb | 2 | 9 | ||
D107 | C0079 | 1 | EcoRI | SpeI | FI | 4402 pb | 779 pb | 5000 pb | 800 pb | 2 | 10 |
2 | XbaI | PstI | BI | 4003 pb | 778 pb | 5000 pb | 800 pb | 2 | 11 | ||
D108 | C0179 | 1 | EcoRI | SpeI | FI | 4402 pb | 746 pb | 2000 pb | 800 pb | 2 | 12 |
2 | XbaI | PstI | BI | 4403 pb | 745 pb | 2000 pb | 800 pb | 3 | 2 | ||
D109 | E0030 | 1 | EcoRI | SpeI | FI | 3166 pb | 746 pb | 3000 pb | 700 pb | 3 | 3 |
2 | XbaI | PstI | BI | 3167 pb | 745 pb | 3000 pb | 700 pb | 3 | 4 | ||
D110 | E0040 | 1 | EcoRI | SpeI | FI | 2056 pb | 743 pb | 2000 pb | 700 pb | 3 | 5 |
2 | XbaI | PstI | BI | 2057 pb | 742 pb | 2000 pb | 700 pb | 3 | 6 | ||
D111 | E1010 | 1 | EcoRI | SpeI | FI | 4402 pb | 704 pb | 5000 pb | 700 pb | 3 | 7 |
2 | XbaI | PstI | BI | 4403 pb | 703 pb | 5000 pb | 700 pb | 3 | 8 | ||
D116 | J23100 | 1 | SpeI | PstI | BV | 2100 pb | 883 pb | 2000 pb | 900 pb | 3 | 9 |
2 | 3 | 10 | |||||||||
D117 | J23107 | 1 | SpeI | PstI | BV | 2100 pb | 883 pb | 2000 pb | 900 pb | 3 | 11 |
2 | 3 | 12 | |||||||||
D118 | B0015 | 1 | EcoRI | XbaI | FV | 3303 pb | 15 pb | 3000 pb | - | 4 | 2 |
2 | 4 | 3 | |||||||||
D119 | I0500 | 1 | SpeI | PstI | FV | 5621 pb | 14 pb | 6000 pb | - | 4 | 4 |
2 | 4 | 5 | |||||||||
D120 | B0030 | 1 | XbaI | PstI | BI | 2057 pb | 37 pb | 2000 pb | 50 pb | 6 | 4 |
2 | 6 | 5 | |||||||||
3 | EcoRI | XbaI | FV | 2079 pb | 15 pb | 2200 pb | - | 4 | 6 | ||
4 | SpeI | PstI | BV | 2080 pb | 14 pb | 2000 pb | - | 4 | 7 | ||
5 | 4 | 8 | |||||||||
D121 | E0422 | 1 | XbaI | PstI | BI | 2057 pb | 939 pb | 2000 pb | 1100 pb | 4 | 9 |
2 | 4 | 10 | |||||||||
3 | 4 | 11 | |||||||||
D122 | E0 | 1 | XbaI | PstI | BI | 2057 pb | 900 pb | 2000 pb | 1000 pb | 4 | 12 |
2 | 2000 pb | 900 pb | 5 | 2 | |||||||
3 | 5 | 3 |
==> conclusion : all the digestion have succeed.....GREAT !