Team:Paris/July 27

From 2008.igem.org

(Difference between revisions)
(New page: === Results of extraction : Electrophoresis === conditions : * 10µl of ladder 1 kb * 2µl of extraction added with 2µl of loading Dye 6x * migration ~30min at 100W * Gel 1, 2, 3, 4...)
(→Results of extraction : Electrophoresis)
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=== Results of extraction : Electrophoresis ===
=== Results of extraction : Electrophoresis ===
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gel2 [[Image:080728gel_2.jpg| gel2|200px]]
gel2 [[Image:080728gel_2.jpg| gel2|200px]]
gel3 [[Image:080728gel_3.jpg| gel3|200px]]
gel3 [[Image:080728gel_3.jpg| gel3|200px]]
-
gel4 [[Image:080726gel_4.jpg| gel4|200px]]<br>
 
-
 
-
gel5 [[Image:‎080726gel_5.jpg| gel5|100px]]
 
-
gel6 [[Image:‎080726gel_6.jpg| gel6|100px]]
 
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|align=center|'''Enz 2'''
|align=center|'''Enz 2'''
|align=center|'''Obs'''
|align=center|'''Obs'''
-
|align=center|'''Exp Size Matrix'''
+
|align=center|'''Exp Size'''
-
|align=center|'''Exp Size BB'''
+
|align=center|'''Mea Size '''
-
|align=center|'''Mea Size Matrix'''
+
-
|align=center|'''Mea Size BB'''
+
|align=center|'''Gel'''
|align=center|'''Gel'''
|align=center|'''Band'''
|align=center|'''Band'''
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! rowspan="2"| BI
! rowspan="2"| BI
! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
-
! rowspan="2"| 34 pb
+
! rowspan="1"|  
-
! rowspan="2"| 1800 pb
+
! rowspan="1"|  
-
! rowspan="2"| 40 pb
+
! rowspan="1"|  
-
! rowspan="1"| 6
+
-
! rowspan="1"| 2
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="1"| 6
+
! rowspan="1"|  
-
! rowspan="1"| 3
+
! rowspan="1"|  
|-
|-
|align=center|3
|align=center|3
Line 58: Line 48:
|align=center| FV
|align=center| FV
|align=center| 2076 pb
|align=center| 2076 pb
-
|align=center| 15 pb
+
|align=center|  
-
|align=center| 2000 pb
+
-
|align=center| -
+
|align=center| 1
|align=center| 1
|align=center| 2
|align=center| 2
Line 69: Line 57:
! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2077 pb
! rowspan="2"| 2077 pb
-
! rowspan="2"| 14 pb
+
! rowspan="2"|  
-
! rowspan="2"| 2000 pb
+
-
! rowspan="2"| -
+
|align=center| 1
|align=center| 1
|align=center| 3
|align=center| 3
Line 86: Line 72:
! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2100 pb
! rowspan="2"| 2100 pb
-
! rowspan="2"| 883 pb
+
! rowspan="2"|  
-
! rowspan="2"| 2000 pb
+
-
! rowspan="2"| 700 pb
+
|align=center| 1
|align=center| 1
|align=center| 5
|align=center| 5
|-
|-
|align=center|2
|align=center|2
-
|align=center| 1
+
|align=center|  
-
|align=center| 6
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;" | D102
! rowspan="2" style="background: #ccccff;" | D102
Line 103: Line 87:
! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2100 pb
! rowspan="2"| 2100 pb
-
! rowspan="2"| 883 pb
+
! rowspan="2"|  
-
! rowspan="2"| 2000 pb
+
! rowspan="1"|  
-
! rowspan="2"| 700 pb
+
! rowspan="1"|  
-
! rowspan="1"| 1
+
-
! rowspan="1"| 7
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="1"| 1
+
! rowspan="1"|  
-
! rowspan="1"| 8
+
! rowspan="1"|  
|-
|-
! rowspan="2" style="background: #ccccff;" | D103
! rowspan="2" style="background: #ccccff;" | D103
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! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2222 pb
! rowspan="2"| 2222 pb
-
! rowspan="2"| 14 pb
+
! rowspan="2"|  
-
! rowspan="2"| 2000 pb
+
|align=center|  
-
! rowspan="2"| -
+
|align=center|  
-
|align=center| 1
+
-
|align=center| 9
+
|-
|-
|align=center|2
|align=center|2
-
|align=center| 1
+
|align=center|  
-
|align=center| 10
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;" | D104
! rowspan="2" style="background: #ccccff;" | D104
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! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2119 pb
! rowspan="2"| 2119 pb
-
! rowspan="2"| 14 pb
+
! rowspan="2"|  
-
! rowspan="2"| 2000 pb
+
|align=center|  
-
! rowspan="2"| -
+
|align=center|  
-
|align=center| 1
+
-
|align=center| 11
+
|-
|-
|align=center|2
|align=center|2
-
|align=center| 1
+
|align=center|  
-
|align=center| 12
+
|align=center|  
|-
|-
! rowspan="4" style="background: #ccccff;" | D105
! rowspan="4" style="background: #ccccff;" | D105
Line 154: Line 132:
! rowspan="1"| BV
! rowspan="1"| BV
! rowspan="1"| 2750 pb
! rowspan="1"| 2750 pb
-
! rowspan="1"| 14 pb
+
|align=center|  
-
|align=center| 3000 pb
+
|align=center|  
-
|align=center| -
+
|align=center|  
-
|align=center| 2
+
-
|align=center| 5
+
|-
|-
|align=center|2
|align=center|2
Line 164: Line 140:
! rowspan="2"| PstI
! rowspan="2"| PstI
! rowspan="2"| BI
! rowspan="2"| BI
-
! rowspan="2"| 2057 pb
 
! rowspan="2"| 707 pb
! rowspan="2"| 707 pb
-
! rowspan="2"| 2000 pb
+
! rowspan="2"|  
-
! rowspan="2"| 700 pb
+
|align=center|  
-
|align=center| 2
+
|align=center|  
-
|align=center| 2
+
|-
|-
|align=center|3
|align=center|3
-
|align=center| 2
+
|align=center|  
-
|align=center| 3
+
|align=center|  
|-
|-
|align=center|4
|align=center|4
-
| align=center|EcoRI
+
|align=center|EcoRI
-
| align=center|SpeI
+
|align=center|SpeI
-
| align=center|FI
+
|align=center|FI
-
| align=center| 2056 pb
+
|align=center| 708 pb
-
| align=center| 708 pb
+
|align=center|  
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 2
+
-
|align=center| 4
+
|-
|-
! rowspan="4" style="background: #ccccff;" | D106
! rowspan="4" style="background: #ccccff;" | D106
! rowspan="4" style="background: #ccccff;" | S03879
! rowspan="4" style="background: #ccccff;" | S03879
|align=center|1
|align=center|1
-
| align=center|SpeI
+
|align=center|SpeI
-
| align=center|PstI
+
|align=center|PstI
-
| align=center|BV
+
|align=center|BV
-
| align=center| 2768 pb
+
|align=center| 2768 pb
-
| align=center|14 pb
+
|align=center|  
-
|align=center| 2700 pb
+
|align=center|  
-
|align=center| -
+
|align=center|  
-
|align=center| 2
+
-
|align=center| 6
+
|-
|-
|align=center|2
|align=center|2
Line 203: Line 173:
! rowspan="2"| PstI
! rowspan="2"| PstI
! rowspan="2"| BI
! rowspan="2"| BI
-
! rowspan="2"| 2057 pb
 
! rowspan="2"| 725 pb
! rowspan="2"| 725 pb
-
! rowspan="2"| 2000 pb
+
! rowspan="2"|  
-
! rowspan="2"| 700 pb
+
|align=center|  
-
|align=center| 2
+
|align=center|  
-
|align=center| 7
+
|-
|-
|align=center|3
|align=center|3
-
|align=center| 2
+
|align=center|  
-
|align=center| 8
+
|align=center|  
|-
|-
|align=center|4
|align=center|4
-
| align=center|EcoRI
+
|align=center|EcoRI
-
| align=center|SpeI
+
|align=center|SpeI
-
| align=center|FI
+
|align=center|FI
-
| align=center| 2756 pb
+
|align=center| 726 pb
-
| align=center| 726 pb
+
|align=center|  
-
|align=center| 3000 pb
+
|align=center|
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 2
+
-
|align=center| 9
+
|-
|-
! rowspan="2" style="background: #ccccff;" |D107
! rowspan="2" style="background: #ccccff;" |D107
Line 231: Line 197:
|align=center|SpeI
|align=center|SpeI
|align=center|FI
|align=center|FI
-
|align=center| 4402 pb
 
|align=center| 779 pb
|align=center| 779 pb
-
|align=center| 5000 pb
+
|align=center|  
-
|align=center| 800 pb
+
|align=center|  
-
|align=center| 2
+
|align=center|  
-
|align=center| 10
+
|-
|-
|align=center| 2
|align=center| 2
Line 242: Line 206:
|align=center|PstI
|align=center|PstI
|align=center|BI
|align=center|BI
-
|align=center| 4003 pb
 
|align=center| 778 pb
|align=center| 778 pb
-
|align=center| 5000 pb
+
|align=center|  
-
|align=center| 800 pb
+
|align=center|  
-
|align=center| 2
+
|align=center|  
-
|align=center| 11
+
|-
|-
! rowspan="2" style="background: #ccccff;"|D108
! rowspan="2" style="background: #ccccff;"|D108
Line 255: Line 217:
|align=center| SpeI
|align=center| SpeI
|align=center| FI
|align=center| FI
-
|align=center| 4402 pb
 
|align=center| 746 pb
|align=center| 746 pb
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 800 pb
+
|align=center|  
-
|align=center| 2
+
|align=center|  
-
|align=center| 12
+
|-
|-
|align=center| 2
|align=center| 2
Line 266: Line 226:
|align=center| PstI
|align=center| PstI
|align=center| BI
|align=center| BI
-
|align=center| 4403 pb
 
|align=center| 745 pb
|align=center| 745 pb
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 800 pb
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 2
+
|-
|-
! rowspan="2" style="background: #ccccff;"|D109
! rowspan="2" style="background: #ccccff;"|D109
Line 279: Line 237:
|align=center|SpeI
|align=center|SpeI
|align=center|FI
|align=center|FI
-
|align=center| 3166 pb
 
|align=center| 746 pb
|align=center| 746 pb
-
|align=center| 3000 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 3
+
|-
|-
|align=center| 2
|align=center| 2
Line 290: Line 246:
|align=center| PstI
|align=center| PstI
|align=center| BI
|align=center| BI
-
|align=center| 3167 pb
 
|align=center| 745 pb
|align=center| 745 pb
-
|align=center| 3000 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 4
+
|-
|-
! rowspan="2" style="background: #ccccff;"|D110
! rowspan="2" style="background: #ccccff;"|D110
Line 303: Line 257:
|align=center| SpeI
|align=center| SpeI
|align=center| FI
|align=center| FI
-
|align=center| 2056 pb
 
|align=center| 743 pb
|align=center| 743 pb
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 5
+
|-
|-
|align=center| 2
|align=center| 2
Line 314: Line 266:
|align=center| PstI
|align=center| PstI
|align=center| BI
|align=center| BI
-
|align=center| 2057 pb
 
|align=center| 742 pb
|align=center| 742 pb
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 6
+
|-
|-
! rowspan="2" style="background: #ccccff;"|D111
! rowspan="2" style="background: #ccccff;"|D111
Line 327: Line 277:
|align=center| SpeI
|align=center| SpeI
|align=center| FI
|align=center| FI
-
|align=center| 4402 pb
 
|align=center| 704 pb
|align=center| 704 pb
-
|align=center| 5000 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 7
+
|-
|-
|align=center| 2
|align=center| 2
Line 338: Line 286:
|align=center| PstI
|align=center| PstI
|align=center| BI
|align=center| BI
-
|align=center| 4403 pb
 
|align=center| 703 pb
|align=center| 703 pb
-
|align=center| 5000 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 8
+
|-
|-
! rowspan="2" style="background: #ccccff;"|D116
! rowspan="2" style="background: #ccccff;"|D116
Line 352: Line 298:
! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2100 pb
! rowspan="2"| 2100 pb
-
! rowspan="2"| 883 pb
+
! rowspan="2"|  
-
! rowspan="2"| 2000 pb
+
! rowspan="1"|  
-
! rowspan="2"| 900 pb
+
! rowspan="1"|  
-
! rowspan="1"| 3
+
-
! rowspan="1"| 9
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="1"| 3
+
! rowspan="1"|  
-
! rowspan="1"| 10
+
! rowspan="1"|  
|-
|-
! rowspan="2" style="background: #ccccff;"|D117
! rowspan="2" style="background: #ccccff;"|D117
Line 369: Line 313:
! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2100 pb
! rowspan="2"| 2100 pb
-
! rowspan="2"| 883 pb
+
! rowspan="2"|  
-
! rowspan="2"| 2000 pb
+
! rowspan="1"|  
-
! rowspan="2"| 900 pb
+
! rowspan="1"|  
-
! rowspan="1"| 3
+
-
! rowspan="1"| 11
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="1"| 3
+
! rowspan="1"|  
-
! rowspan="1"| 12
+
! rowspan="1"|  
|-
|-
! rowspan="2" style="background: #ccccff;"|D118
! rowspan="2" style="background: #ccccff;"|D118
Line 386: Line 328:
! rowspan="2"| FV
! rowspan="2"| FV
! rowspan="2"| 3303 pb
! rowspan="2"| 3303 pb
-
! rowspan="2"| 15 pb
+
! rowspan="2"|  
-
! rowspan="2"| 3000 pb
+
! rowspan="1"|  
-
! rowspan="2"| -
+
! rowspan="1"|  
-
! rowspan="1"| 4
+
-
! rowspan="1"| 2
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="1"| 4
+
! rowspan="1"|  
-
! rowspan="1"| 3
+
! rowspan="1"|  
|-
|-
! rowspan="2" style="background: #ccccff;"|D119
! rowspan="2" style="background: #ccccff;"|D119
Line 403: Line 343:
! rowspan="2"| FV
! rowspan="2"| FV
! rowspan="2"| 5621 pb
! rowspan="2"| 5621 pb
-
! rowspan="2"| 14 pb 
+
! rowspan="2"|  
-
! rowspan="2"| 6000 pb
+
! rowspan="1"|  
-
! rowspan="2"| -
+
! rowspan="1"|  
-
! rowspan="1"| 4
+
-
! rowspan="1"| 4
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="1"| 4
+
! rowspan="1"|  
-
! rowspan="1"| 5
+
! rowspan="1"|  
|-
|-
! rowspan="5" style="background: #ccccff;" | D120
! rowspan="5" style="background: #ccccff;" | D120
Line 419: Line 357:
! rowspan="2"| PstI
! rowspan="2"| PstI
! rowspan="2"| BI
! rowspan="2"| BI
-
! rowspan="2"| 2057 pb
 
! rowspan="2"| 37 pb
! rowspan="2"| 37 pb
-
! rowspan="2"| 2000 pb
+
! rowspan="2"|  
-
! rowspan="2"| 50 pb
+
! rowspan="1"|  
-
! rowspan="1"| 6
+
! rowspan="1"|  
-
! rowspan="1"| 4
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="1"| 6
+
! rowspan="1"|  
-
! rowspan="1"| 5
+
! rowspan="1"|  
|-
|-
|align=center|3
|align=center|3
Line 435: Line 371:
! rowspan="1"|  FV
! rowspan="1"|  FV
! rowspan="1"| 2079 pb
! rowspan="1"| 2079 pb
-
! rowspan="1"| 15 pb
+
! rowspan="1"|  
-
! rowspan="1"| 2200 pb
+
! rowspan="1"|  
-
! rowspan="1"| -
+
! rowspan="1"|  
-
! rowspan="1"| 4
+
-
! rowspan="1"| 6
+
|-
|-
|align=center|4
|align=center|4
Line 446: Line 380:
! rowspan="2"| BV
! rowspan="2"| BV
! rowspan="2"| 2080 pb
! rowspan="2"| 2080 pb
-
! rowspan="2"| 14 pb
+
! rowspan="2"|  
-
! rowspan="2"| 2000 pb
+
! rowspan="1"|  
-
! rowspan="2"| -
+
! rowspan="1"|  
-
! rowspan="1"| 4
+
-
! rowspan="1"| 7
+
|-
|-
|align=center|5
|align=center|5
-
! rowspan="1"| 4
+
! rowspan="1"|  
-
! rowspan="1"| 8
+
! rowspan="1"|  
|-
|-
! rowspan="3" style="background: #ccccff;"|D121
! rowspan="3" style="background: #ccccff;"|D121
Line 462: Line 394:
! rowspan="3"| PstI
! rowspan="3"| PstI
! rowspan="3"| BI
! rowspan="3"| BI
-
! rowspan="3"| 2057 pb
 
! rowspan="3"| 939 pb
! rowspan="3"| 939 pb
-
! rowspan="3"| 2000 pb
+
! rowspan="3"|  
-
! rowspan="3"| 1100 pb
+
! rowspan="1"|  
-
! rowspan="1"| 4
+
! rowspan="1"|  
-
! rowspan="1"| 9
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="1"| 4
+
! rowspan="1"|  
-
! rowspan="1"| 10
+
! rowspan="1"|  
|-
|-
|align=center|3
|align=center|3
-
! rowspan="1"| 4
+
! rowspan="1"|  
-
! rowspan="1"| 11
+
! rowspan="1"|  
|-
|-
! rowspan="3" style="background: #ccccff;"|D122
! rowspan="3" style="background: #ccccff;"|D122
-
! rowspan="3" style="background: #ccccff;"|E0
+
! rowspan="3" style="background: #ccccff;"|E0840
|align=center|1
|align=center|1
! rowspan="3"| XbaI
! rowspan="3"| XbaI
! rowspan="3"| PstI
! rowspan="3"| PstI
! rowspan="3"| BI
! rowspan="3"| BI
-
! rowspan="3"| 2057 pb
 
! rowspan="3"| 900 pb
! rowspan="3"| 900 pb
-
! rowspan="1"| 2000 pb
+
! rowspan="1"|  
-
! rowspan="1"| 1000 pb
+
! rowspan="1"|  
-
! rowspan="1"| 4
+
! rowspan="1"|  
-
! rowspan="1"|  12
+
|-
|-
|align=center|2
|align=center|2
-
! rowspan="2"| 2000 pb
+
! rowspan="1"|  
-
! rowspan="2"| 900 pb
+
! rowspan="1"|  
-
! rowspan="1"| 5
+
-
! rowspan="1"| 2
+
|-
|-
|align=center|3
|align=center|3
-
! rowspan="1"| 5
+
! rowspan="1"|  
-
! rowspan="1"| 3
+
! rowspan="1"|  
|}
|}
-
==> conclusion : all the digestion have succeed.....GREAT !
+
==> conclusion :

Revision as of 14:42, 28 July 2008

Results of extraction : Electrophoresis

conditions :

  • 10µl of ladder 1 kb
  • 2µl of extraction added with 2µl of loading Dye 6x
  • migration ~30min at 100W
  • Gel 1, 2, 3, 4, 5 = 0.8%
  • Gel 6 = 1,5%


gel1 gel1 gel2 gel2 gel3 gel3



Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Mea Size Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb
2
3 EcoRI XbaI FV 2076 pb 1 2
4 SpeI PstI BV 2077 pb 1 3
5 1 4
D101 J23101 1 SpeI PstI BV 2100 pb 1 5
2
D102 J23109 1 SpeI PstI BV 2100 pb
2
D103 R0079 1 SpeI PstI BV 2222 pb
2
D104 R0040 1 SpeI PstI BV 2119 pb
2
D105 S03154 1 SpeI PstI BV 2750 pb
2 XbaI PstI BI 707 pb
3
4 EcoRI SpeI FI 708 pb
D106 S03879 1 SpeI PstI BV 2768 pb
2 XbaI PstI BI 725 pb
3
4 EcoRI SpeI FI 726 pb
D107 C0079 1 EcoRI SpeI FI 779 pb
2 XbaI PstI BI 778 pb
D108 C0179 1 EcoRI SpeI FI 746 pb
2 XbaI PstI BI 745 pb
D109 E0030 1 EcoRI SpeI FI 746 pb
2 XbaI PstI BI 745 pb
D110 E0040 1 EcoRI SpeI FI 743 pb
2 XbaI PstI BI 742 pb
D111 E1010 1 EcoRI SpeI FI 704 pb
2 XbaI PstI BI 703 pb
D116 J23100 1 SpeI PstI BV 2100 pb
2
D117 J23107 1 SpeI PstI BV 2100 pb
2
D118 B0015 1 EcoRI XbaI FV 3303 pb
2
D119 I0500 1 SpeI PstI FV 5621 pb
2
D120 B0030 1 XbaI PstI BI 37 pb
2
3 EcoRI XbaI FV 2079 pb
4 SpeI PstI BV 2080 pb
5
D121 E0422 1 XbaI PstI BI 939 pb
2
3
D122 E0840 1 XbaI PstI BI 900 pb
2
3


==> conclusion :

Retrieved from "http://2008.igem.org/Team:Paris/July_27"