"
Alberta NINT/25 July 2008
From 2008.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
V = volume of culture used in the assay in mLs | V = volume of culture used in the assay in mLs | ||
A600 is detection of cell density | A600 is detection of cell density | ||
| - | Alberta_NINT_LacZ_assay_July_24.xls | + | [[Image:Alberta_NINT_LacZ_assay_July_24.xls]] |
[[Alberta_NINT/28_July_2008 | Next entry >]] | [[Alberta_NINT/28_July_2008 | Next entry >]] | ||
Revision as of 19:21, 29 July 2008
lab work (SD):
Some yellow color developed overnight so 50 ul of Na2CO3 was added to stop the reaction. The A550
and A420 readings were taken. Miller units were calculated using the formula
Miller Units = 1000 x [(A420 - 1.75 x A550] / (T x V x 4 x A600)
where A420 is detection of yellow
A550 is detection of cell debris
T = time of reaction in minutes
V = volume of culture used in the assay in mLs
A600 is detection of cell density
File:Alberta NINT LacZ assay July 24.xls
