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User:University of Washington/29 July 2008
From 2008.igem.org
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== luxR from pLac == | == luxR from pLac == | ||
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-Restriction digests of R0010 and E0240 started; will incubate at 37 overnight. | -Restriction digests of R0010 and E0240 started; will incubate at 37 overnight. | ||
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| + | ==LuxR from AraC and TetR== | ||
| + | |||
| + | -PCR purified the AraC and TetR promoter. | ||
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| + | -Digest the Elowitz's plasmid with enzyme HindIII to find out what plasmid exactly the strain contain. Two potential >>(pCS26 has 4 sites: expected 4716, 1990, 1982, 785 bp) (pACYC184 has 1 site: 4245 bp) | ||
| + | * 34.5ul ddH2O + 5ul NEB2 + 10ul DNA(assume 100 ng/ul) + ...vortex... + 0.5 ul HindIII +....centrifuge ... | ||
| + | * incubated 37 degree Celsius for 1.5 hours | ||
| + | |||
| + | -Ran gel. | ||
| + | |||
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Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 23:57, 29 July 2008
luxR from pLac
-Bacterial stab of part I0462 received from iGEM HQ.
-Sequence of R0010+E0240 ligation received back and found to be incorrect. Assembly of these parts must be retried.
-Restriction digests of R0010 and E0240 started; will incubate at 37 overnight.
LuxR from AraC and TetR
-PCR purified the AraC and TetR promoter.
-Digest the Elowitz's plasmid with enzyme HindIII to find out what plasmid exactly the strain contain. Two potential >>(pCS26 has 4 sites: expected 4716, 1990, 1982, 785 bp) (pACYC184 has 1 site: 4245 bp)
- 34.5ul ddH2O + 5ul NEB2 + 10ul DNA(assume 100 ng/ul) + ...vortex... + 0.5 ul HindIII +....centrifuge ...
- incubated 37 degree Celsius for 1.5 hours
-Ran gel.
Back to Team:University_of_Washington/Notebook#Notebook
