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Illinois/1 June 2008
From 2008.igem.org
(Difference between revisions)
(New page: *Receptor Tyrosine Kinase method: Made Yeast Media YPD Medium, per liter: 10g yeast extract 20g peptone 20g dextrose 20g agar(only for plates) 1. Dextrose filter sterilized, the rest aut...) |
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*Receptor Tyrosine Kinase method: | *Receptor Tyrosine Kinase method: | ||
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Made Yeast Media | Made Yeast Media | ||
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YPD Medium, per liter: | YPD Medium, per liter: | ||
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10g yeast extract | 10g yeast extract | ||
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20g peptone | 20g peptone | ||
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20g dextrose | 20g dextrose | ||
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20g agar(only for plates) | 20g agar(only for plates) | ||
1. Dextrose filter sterilized, the rest autoclaved | 1. Dextrose filter sterilized, the rest autoclaved | ||
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2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve | 2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve | ||
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3. Dextrose added to autoclaved media to equivalent of 20 g/L | 3. Dextrose added to autoclaved media to equivalent of 20 g/L | ||
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4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes | 4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes | ||
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5. Poured into plates and allowed to solidify | 5. Poured into plates and allowed to solidify | ||
Revision as of 15:38, 30 July 2008
- Receptor Tyrosine Kinase method:
Made Yeast Media
YPD Medium, per liter:
10g yeast extract
20g peptone
20g dextrose
20g agar(only for plates)
1. Dextrose filter sterilized, the rest autoclaved
2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve
3. Dextrose added to autoclaved media to equivalent of 20 g/L
4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes
5. Poured into plates and allowed to solidify
