From 2008.igem.org
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| - | *Receptor Tyrosine Kinase method:
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| - | Made Yeast Media
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| - | YPD Medium, per liter:
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| - | 10g yeast extract
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| - | 20g peptone
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| - | 20g dextrose
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| - | 20g agar(only for plates)
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| - | 1. Dextrose filter sterilized, the rest autoclaved
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| - | 2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve
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| - | 3. Dextrose added to autoclaved media to equivalent of 20 g/L
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| - | 4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes
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| - | 5. Poured into plates and allowed to solidify
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Latest revision as of 15:39, 30 July 2008
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