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Minnesota/30 July 2008
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Gel electrophoresis was performed to separate digested LacI/Lambda cI dually-repressed promoter from the plasmid backbone. Lanes (L -> R) contain: 1kb ladder, two lanes of promoter digest, and a 100 bp ladder. | Gel electrophoresis was performed to separate digested LacI/Lambda cI dually-repressed promoter from the plasmid backbone. Lanes (L -> R) contain: 1kb ladder, two lanes of promoter digest, and a 100 bp ladder. | ||
[[Image:Gel7.30.08.jpg|500px||center|thumb|Electrophoretic gel run on 7.30.08]] | [[Image:Gel7.30.08.jpg|500px||center|thumb|Electrophoretic gel run on 7.30.08]] | ||
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| + | |Gel Purification - purified the DNA bands from the gel to extract only DNA and purify out cell debris. | ||
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| + | |Ligation Reaction - Ligated BV + LacI/LAMBDAcI together. After following ligation table, allow to incubate @ 16C for 45 minutes. Then heat inactivate enzyme in 65C water bath for 15 minutes. Follow the table below: | ||
Revision as of 19:39, 30 July 2008
Gel electrophoresis was performed to separate digested LacI/Lambda cI dually-repressed promoter from the plasmid backbone. Lanes (L -> R) contain: 1kb ladder, two lanes of promoter digest, and a 100 bp ladder.
|- |Gel Purification - purified the DNA bands from the gel to extract only DNA and purify out cell debris. |- |Ligation Reaction - Ligated BV + LacI/LAMBDAcI together. After following ligation table, allow to incubate @ 16C for 45 minutes. Then heat inactivate enzyme in 65C water bath for 15 minutes. Follow the table below:
