"
Minnesota/31 July 2008
From 2008.igem.org
(Difference between revisions)
Emartin9808 (Talk | contribs)
(New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 30)'''|| width=158|'''[[...)
Newer edit →
(New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 30)'''|| width=158|'''[[...)
Newer edit →
Revision as of 15:47, 31 July 2008
| Back to Notebook Home | |
| Go to Previous Day (July 30) | Go to Next Day (August 1) |
| 1. Pick Colonies from BV:Lac/LAMBDA (dual promoter and base vector): Picked colonies and placed BV:dualpromoters in 2mL LB cultures to incubate @ 37C for 8-12 hours. |
| 2. Plasmid Prep (1) BV:Tet/p22:RFP and (2) BV:Tetpromoter:LAMBDAcIgene: Using QIAmini prep guidelines - prepped 5 samples of BV:TetR/p22:RFP and 5 samples of BV:TetRpromoter:LAMBDAcIgene. Steps: |
| NOTE: Last step of Plasmid Prep was performed differently to obtain a higher concentration of DNA. After elution buffer fell through and flow through had DNA in it so repoured that into spin column a second time to spin again for another 60 seconds to extract more DNA. |
| 3. Spectrophotometry: Spec the 10 plasmid prep samples to check for concentration of DNA. |
