Wisconsin: Lignin Project/18 July 2008
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(New page: == Team Sorbitol == Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD. They were then stored at -80. Primers were then designed and ordered to insert '...)
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(New page: == Team Sorbitol == Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD. They were then stored at -80. Primers were then designed and ordered to insert '...)
Newer edit →
Revision as of 20:40, 31 July 2008
Team Sorbitol
Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD.
They were then stored at -80.
Primers were then designed and ordered to insert srlD into the pET28a vector which will provide a His tag that can be used to purify the enzyme.