Team:NTU-Singapore/Notebook/25 July 2008

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Revision as of 01:39, 1 August 2008

  • Darius
    • Miniprep 23 tubes of bacteria, E7-ter, lysis, lsrA respectively.
  • Digestion of E7-terminator, Lysis Standard Vector, LsrA standard vector.
  • Run Gel Check
    • E7 showed a 2k Band, however, this is not conclusive as GFP vector and E7 Is of the same size.

Lysis standard vector is successful, showed a band between 100bp and 200bp(140bp) LsrA standard vector failed.

  • PCR verification of E7 terminator. Ran PCR with different constituents.
    • Old E7 Primers, New E7 primers, E7 verification primers.
  • Results seen on saturday morning.