Team:NTU-Singapore/Notebook/26 July 2008
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(New page: * Darius ** Did a gel run for Lysis terminator. However, unsuccessful gel showed a band between 100bp and 200bp. Correct size of Lysis terminator should be 300bp. *miniprep another 12 tube...)
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(New page: * Darius ** Did a gel run for Lysis terminator. However, unsuccessful gel showed a band between 100bp and 200bp. Correct size of Lysis terminator should be 300bp. *miniprep another 12 tube...)
Newer edit →
Revision as of 01:55, 1 August 2008
- Darius
- Did a gel run for Lysis terminator.
However, unsuccessful gel showed a band between 100bp and 200bp. Correct size of Lysis terminator should be 300bp.
- miniprep another 12 tubes of LsrA standard vector innoculated yesterday.
- digestion of the plasmid extracted.
- Gel run check
- digestion of the plasmid extracted.
LsrA is successful. band at 140bp. Though abnormality similar to Lysis, a double band is observed. 110bp and 150bp respectively, very close to each other( estimation of values)