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Minnesota/1 August 2008
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|'''1. Ran the terminator digest out on a gel:''' After which we were unable to see a band the appropriate length, so we simply cut out the gel where we expected the band to be (terminator is very small - so hard to see). Gel purified - then used this in our next ligation. When spec'ed the purified gel terminator product, there were 2.5ngram/uL of DNA concentration. | |'''1. Ran the terminator digest out on a gel:''' After which we were unable to see a band the appropriate length, so we simply cut out the gel where we expected the band to be (terminator is very small - so hard to see). Gel purified - then used this in our next ligation. When spec'ed the purified gel terminator product, there were 2.5ngram/uL of DNA concentration. | ||
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| - | |'''2. Currently have two parts that are base vector-promoter-gene, and one that is base vector-promoter:''' We are currently ligating the terminator to the two parts that have vector-promoter-gene. | + | |'''2. Currently have two parts that are base vector-promoter-gene, and one that is base vector-promoter:''' We are currently ligating the terminator to the two parts that have vector-promoter-gene. |
| + | |- | ||
| + | |The base vector-promoter-gene constructs are: TetR promoter:Lambda cI and Tet/p22 promoter:RFP. | ||
| + | |- | ||
| + | |The base vector-promoter construct is: Lambda cI/Lac promoter | ||
Revision as of 18:48, 4 August 2008
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| 1. Ran the terminator digest out on a gel: After which we were unable to see a band the appropriate length, so we simply cut out the gel where we expected the band to be (terminator is very small - so hard to see). Gel purified - then used this in our next ligation. When spec'ed the purified gel terminator product, there were 2.5ngram/uL of DNA concentration. |
| 2. Currently have two parts that are base vector-promoter-gene, and one that is base vector-promoter: We are currently ligating the terminator to the two parts that have vector-promoter-gene. |
| The base vector-promoter-gene constructs are: TetR promoter:Lambda cI and Tet/p22 promoter:RFP. |
| The base vector-promoter construct is: Lambda cI/Lac promoter |
