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Team:ESBS-Strasbourg/4 August 2008
From 2008.igem.org
(Difference between revisions)
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Construction of the operator BioBricks (GAL4O, LexAO, LacO, CIO, TetO)<br> | Construction of the operator BioBricks (GAL4O, LexAO, LacO, CIO, TetO)<br> | ||
* hybridisation of the ordered oligos | * hybridisation of the ordered oligos | ||
| - | * ligation into the XbaI, PstI cutted vector pSB1A2 | + | * ligation into the XbaI, PstI cutted vector pSB1A2 ''(using protocol 2c)'' |
| - | * transformation into competent TOP10 and plating on LB-Amp | + | * transformation into competent TOP10 and plating on LB-Amp ''(using the heat-shock protocol)'' |
== General == | == General == | ||
== Quote of the day == | == Quote of the day == | ||
Revision as of 08:06, 5 August 2008
Contents |
DryLab
Modeling
WetLab
Katja
Construction of the operator BioBricks (GAL4O, LexAO, LacO, CIO, TetO)
- hybridisation of the ordered oligos
- ligation into the XbaI, PstI cutted vector pSB1A2 (using protocol 2c)
- transformation into competent TOP10 and plating on LB-Amp (using the heat-shock protocol)
